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| Product Name | Basement Membrane Matrix, High Concentration, Phenol Red-Free, LDEV-Free |
| Catalog No. | RESK-0232 |
| Description | Basement Membrane Matrix, High Concentration, Phenol Red-Free, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG) and nidogen, together with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors naturally present in EHS tumors. At room temperature it polymerizes into a biologically active three-dimensional matrix that mimics the structure, composition, physical properties and function of the in vivo cell basement membrane, facilitating in vitro cell culture and differentiation and enabling studies of cell morphology, biochemical function, migration, invasion and gene expression. The three-dimensional culture matrix formed by this product promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells and hair follicle cells, and can influence gene expression in 3D cultures of adult mouse hepatocytes and human mammary epithelial cells. It also serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, and a 3D scaffold for transplanted tumor cell growth in animal models. In addition, it supports peripheral nerve regeneration and the differentiation of bovine oviduct epithelial cells. This product is sterile, phenol red-free, and has a protein concentration greater than 18 mg/mL, meeting the requirements of various applications including in vivo tumorigenesis, angiogenesis studies, tumor cell migration and 3D cell research. |
| Intended Use | Used as a three-dimensional culture matrix for in vivo tumorigenesis (xenograft) models, angiogenesis studies, tumor cell migration and invasion assays, and 3D cell culture and differentiation research. For research use. |
| Principle / Technology | A soluble basement membrane preparation extracted from EHS mouse tumors. At room temperature, its extracellular matrix proteins (laminin, collagen IV, HSPG, nidogen) polymerize into a biologically active 3D gel that mimics the native basement membrane. |
| Performance Range / Specifications | Protein concentration: >18 mg/mL Endotoxin: <8 EU/mL LDEV: not detected (LDEV-free) Mycoplasma: not detected Sterility: no bacterial or fungal growth Phenol red: free |
| Reaction Conditions / Protocol | Thawing: place the matrix in an ice box and thaw in a 4°C refrigerator for approximately 3-4 days; the bottle may be gently shaken during thawing to mix. All operations should be performed under sterile conditions, and all items involved should be pre-cooled before use. |
| Components / Formulation | Soluble basement membrane preparation composed mainly of laminin, collagen type IV, heparan sulfate proteoglycan (HSPG) and nidogen, plus growth factors including TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other EHS tumor-derived growth factors. Sterile and phenol red-free. |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 2 years when stored at -20°C. |
| Package Specifications | 5 mL 10 mL |
| Product Form | Frozen liquid that polymerizes into a gel at room temperature |
| Quality Control | Sterilized by rigorous dialysis to guarantee no bacterial or fungal growth; protein concentration >18 mg/mL; endotoxin <8 EU/mL; verified free of LDEV and mycoplasma contamination. |
| Concentration | >18 mg/mL |
| Source / Origin | Extracted from EHS (Engelbreth-Holm-Swarm) mouse tumors rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years when stored at -20°C. |
| Compatibility | Supports attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells and hair follicle cells; suitable for 3D culture of adult mouse hepatocytes and human mammary epithelial cells, tumor cell invasion studies, in vitro/in vivo angiogenesis research, transplanted tumor growth in animal models, peripheral nerve regeneration and bovine oviduct epithelial cell differentiation. |
| Recommended Buffer System | If the thawed matrix does not form a homogeneous slurry (e.g., a jelly-like or semi-solidified state, which may occur due to the high concentration) and shows poor flowability or is difficult to pipette, it can be diluted with pre-cooled DMEM medium or pre-cooled PBS buffer. |
| Application Notes / Precautions | All operations must be performed under sterile conditions, and all items involved should be pre-cooled before handling. Thaw on ice at 4°C for 3-4 days; shake gently during thawing to mix. If the matrix appears gel-like or semi-solidified after overnight thawing at 4°C and is difficult to pipette, dilute with pre-cooled DMEM medium or pre-cooled PBS buffer; cutting off a small section of the pipette tip can also facilitate aspiration. Mainly intended for in vivo tumorigenesis experiments. |
| Batch-to-Batch Consistency | Large-scale production with a monthly output at the 50 L scale or above supports a stable and consistent supply. |
For research use only, not for clinical use.
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