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| Product Name | Basement Membrane Matrix, High Concentration, LDEV-Free |
| Catalog No. | RESK-0228 |
| Description | This high-concentration, LDEV-free basement membrane matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and nidogen, along with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors naturally present in EHS tumors. At room temperature, it polymerizes to form a biologically active three-dimensional matrix that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, facilitating in vitro cell culture and differentiation. It can be used in studies of cell morphology, biochemical function, migration, invasion, and gene expression. The matrix promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. It can influence gene expression in 3D cultures of adult rat hepatocytes and human mammary epithelial cells, serve as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, and a 3D scaffold for transplanted tumor cell growth in in vivo animal models. It also supports peripheral nerve regeneration and the differentiation of bovine oviduct epithelial cells. |
| Intended Use | Suitable for in vivo tumorigenesis (tumor xenograft) assays, angiogenesis research, tumor cell migration studies, and 3D cell culture research. |
| Principle / Technology | Soluble basement membrane preparation extracted from EHS mouse tumors; polymerizes at room temperature to form a biologically active 3D matrix mimicking the in vivo basement membrane. |
| Performance Range / Specifications | Sterility: rigorously dialyzed for sterilization; guaranteed free of bacterial and fungal growth. Protein concentration: > 18 mg/mL. Endotoxin residue: < 8 EU/mL. Safety: free of LDEV residue. High yield: monthly production capacity above the 50 L scale. Mycoplasma: verified free of mycoplasma contamination. |
| Reaction Conditions / Protocol | Thawing: place the matrix in an ice box and thaw in a 4 °C refrigerator for approximately 3–4 days; the matrix may be swirled and mixed during thawing. All operations must be performed under aseptic conditions, and all items involved should be pre-cooled before use. |
| Components / Formulation | Main components: laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and nidogen; also contains growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors inherent to EHS tumors. Sterile preparation. |
| Storage Conditions | Store at -20 °C. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 5 mL 10 mL 1.5 mL |
| Product Form | Frozen liquid; forms a gel (polymerizes) at room temperature. |
| Quality Control | Rigorous dialysis-based sterilization ensuring no bacterial or fungal growth; endotoxin < 8 EU/mL; verified free of mycoplasma contamination; LDEV-free. |
| Concentration | Protein concentration > 18 mg/mL. |
| Source / Origin | Extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C. |
| Compatibility | Compatible with in vivo tumorigenesis assays, angiogenesis studies, tumor cell migration/invasion assays, and 3D cell culture; can be diluted with pre-cooled DMEM medium or pre-cooled PBS buffer. |
| Recommended Buffer System | If the matrix fails to form a homogeneous slurry after thawing (jelly-like or semi-solidified state due to the high-concentration properties), dilute with pre-cooled DMEM medium or pre-cooled PBS buffer. |
| Application Notes / Precautions | All operations must be performed aseptically; all items involved should be pre-cooled before use. Thaw in an ice box in a 4 °C refrigerator for 3–4 days; swirling and mixing during thawing is acceptable. Color variation (pale yellow to dark red) in phenol red-containing matrix is caused by the interaction of phenol red and bicarbonate with CO2; the color difference diminishes after equilibration with 5% CO2. After thawing, gently swirl the bottle to disperse the matrix evenly. If the matrix does not present good flowability after overnight thawing on ice at 4 °C (e.g., jelly-like or semi-solidified state, which is characteristic of high-concentration matrix) and is difficult to pipette, dilute with pre-cooled DMEM medium or pre-cooled PBS buffer; the pipette tip can be cut off at the front end to aid aspiration. |
For research use only, not for clinical use.
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