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| Product Name | Basement Membrane Matrix, High Concentration, GFR, LDEV-Free |
| Catalog No. | RESK-0242 |
| Description | This product is a soluble basement membrane preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumor, which is rich in extracellular matrix proteins. Its main components are laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin/nidogen, together with growth factors such as TGF-beta, EGF, IGF, and FGF. The GFR (growth factor reduced) version is obtained through special processing and effectively supports experiments that require low growth factor conditions, such as signaling pathway and growth factor-related studies. At room temperature, the matrix polymerizes into a biologically active three-dimensional matrix that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, facilitating in vitro cell culture and differentiation, and enabling studies of cell morphology, biochemical function, migration, invasion, and gene expression. |
| Intended Use | Formation of 3D culture matrices that promote adhesion and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells; modulation of gene expression in 3D cultures of adult rat hepatocytes and human mammary epithelial cells; basic scaffold for tumor cell invasion studies; essential matrix for in vitro and in vivo angiogenesis research; 3D scaffold for transplanted tumor cell growth in in vivo animal models (in vivo tumorigenesis assays); support of peripheral nerve regeneration and differentiation of bovine oviduct epithelial cells. |
| Principle / Technology | Soluble basement membrane preparation that polymerizes at room temperature into a biologically active 3D hydrogel mimicking the in vivo basement membrane, providing structural and biochemical cues for cell adhesion, differentiation, migration, and invasion. |
| Sample Type | Cultured cells (epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, hair follicle cells, tumor cells); in vivo animal models |
| Performance Range / Specifications | Protein concentration >18 mg/mL Endotoxin <8 EU/mL LDEV-free Free of bacterial and fungal growth (sterile dialysis) Mycoplasma-free Monthly batch output above the 50 L scale |
| Sensitivity / LOD | Endotoxin: <8 EU/mL |
| Reaction Conditions / Protocol | Thaw by placing the matrix in an ice box in a 4 °C refrigerator for approximately 3-4 days; the matrix can be gently shaken and mixed during thawing. Polymerizes into a 3D gel at room temperature. |
| Components / Formulation | Laminin Collagen type IV Heparan sulfate proteoglycan (HSPG) Entactin/nidogen Growth factors including TGF-beta, EGF, IGF, and FGF (reduced levels in this GFR version) |
| Storage Conditions | Store at -20 °C. |
| Shelf Life | 2 years |
| Package Specifications | 5 mL 10 mL |
| Product Form | Sterile liquid that polymerizes into a 3D gel at room temperature |
| Quality Control | Sterile: rigorously dialyzed and sterilized; guaranteed free of bacterial and fungal growth Protein concentration: >18 mg/mL Endotoxin: <8 EU/mL LDEV-free (no LDEV residue) Mycoplasma: tested and confirmed negative |
| Concentration | >18 mg/mL protein (high concentration) |
| Source / Origin | Engelbreth-Holm-Swarm (EHS) mouse tumor |
| Shipping Conditions | Shipped on dry ice |
| Expiration Date / Stability | Stable for 2 years when stored at -20 °C. |
| Application Notes / Precautions | All operations must be performed under sterile conditions, and all involved items should be pre-cooled before use. Thaw at 4 °C on ice for 3-4 days; if the matrix does not appear homogeneous after thawing (e.g., jelly-like or semi-solidified, a characteristic of high-concentration matrix) or is difficult to pipette, dilute with pre-cooled DMEM medium or pre-cooled PBS buffer; cutting off a small section of the pipette tip can also facilitate aspiration. Color variation (pale yellow to dark red) in phenol red-containing matrix is caused by interaction of phenol red and bicarbonate with CO2; the color difference diminishes after equilibration with 5% CO2. After freeze-thaw, gently shake the bottle to disperse the matrix evenly. |
| Batch-to-Batch Consistency | High production capacity with a monthly batch output above the 50 L scale supports consistent supply. |
For research use only, not for clinical use.
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