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| Product Name | Basement Membrane Matrix, hESC-Qualified, LDEV-Free |
| Catalog No. | RESK-0235 |
| Description | Basement Membrane Matrix, hESC-Qualified, LDEV-Free is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG) and nidogen, together with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other growth factors naturally present in EHS tumors. At room temperature it polymerizes into a biologically active three-dimensional matrix that mimics the structure, composition, physical properties and function of the in vivo cell basement membrane, facilitating in vitro cell culture and differentiation and enabling studies of cell morphology, biochemical function, migration, invasion and gene expression. This matrix can influence gene expression in 3D cultures of adult mouse hepatocytes and human mammary epithelial cells, and serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, and a 3D scaffold for transplanted tumor cell growth in animal models. The product is sterile and has been validated by human embryonic stem cell (hESC) culture to meet the growth requirements of stem cells. |
| Intended Use | Used as a three-dimensional culture matrix for human embryonic stem cell (hESC) and stem cell culture, stem cell-derived organoid culture, tumor cell invasion studies, angiogenesis research and transplanted tumor models. For research use. |
| Principle / Technology | A soluble basement membrane preparation extracted from EHS mouse tumors. At room temperature, its extracellular matrix proteins (laminin, collagen IV, HSPG, nidogen) polymerize into a biologically active 3D gel that mimics the native basement membrane. Each lot is qualified by hESC culture validation. |
| Performance Range / Specifications | Protein concentration: 8-20 mg/mL Endotoxin: <8 EU/mL LDEV: not detected (LDEV-free) Mycoplasma: not detected Sterility: no bacterial or fungal growth Qualification: validated by hESC culture |
| Reaction Conditions / Protocol | All operations should be performed under sterile conditions with pre-cooled items. Thaw the matrix on ice at 4°C and keep it cold during handling. |
| Components / Formulation | Soluble basement membrane preparation composed mainly of laminin, collagen type IV, heparan sulfate proteoglycan (HSPG) and nidogen, plus growth factors including TGF-beta, EGF, IGF, FGF, tissue plasminogen activator and other EHS tumor-derived growth factors. Sterile. |
| Storage Conditions | Store at -20°C. |
| Shelf Life | 2 years when stored at -20°C. |
| Package Specifications | 5 mL 10 mL |
| Product Form | Frozen liquid that polymerizes into a gel at room temperature |
| Quality Control | Sterilized by rigorous dialysis to guarantee no bacterial or fungal growth; protein concentration 8-20 mg/mL; endotoxin <8 EU/mL; verified free of LDEV and mycoplasma contamination; validated by human embryonic stem cell (hESC) culture to confirm the matrix supports stem cell growth. |
| Concentration | 8-20 mg/mL |
| Source / Origin | Extracted from EHS (Engelbreth-Holm-Swarm) mouse tumors rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years when stored at -20°C. |
| Compatibility | Validated for human embryonic stem cell (hESC) culture and suitable for stem cell-derived organoid culture; supports 3D culture of adult mouse hepatocytes and human mammary epithelial cells, tumor cell invasion studies, in vitro/in vivo angiogenesis research and transplanted tumor growth in animal models. |
| Application Notes / Precautions | Perform all operations under sterile conditions with pre-cooled consumables; keep the matrix cold during handling to prevent premature gelation. |
| Batch-to-Batch Consistency | Large-scale production with a monthly batch output at the 50 L scale or above supports a stable and consistent supply. |
For research use only, not for clinical use.
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