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| Product Name | Basement Membrane Matrix, GFR, Phenol Red-Free, LDEV-Free |
| Catalog No. | RESK-0221 |
| Description | This basement membrane matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and nidogen (entactin), together with growth factors such as TGF-beta, EGF, IGF, and FGF. This growth factor-reduced (GFR) version is produced by a special process that lowers growth factor content, making it well suited for experiments that require low growth factor conditions, such as studies of signaling pathways and growth factor-related processes. At room temperature, the matrix polymerizes to form a biologically active three-dimensional scaffold that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, supporting in vitro cell culture and differentiation and enabling studies of cell morphology, biochemical function, migration, invasion, and gene expression. The resulting 3D culture matrix promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells, and can modulate gene expression in 3D cultures of adult rat hepatocytes and human mammary epithelial cells. It also serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, a 3D scaffold for transplanted tumor cell growth in animal models, and supports peripheral nerve regeneration and differentiation of bovine oviduct epithelial cells. The product is sterile, phenol red-free, and supplied at a protein concentration of 8-12 mg/mL to meet a variety of experimental requirements. |
| Intended Use | For research use in experiments requiring low growth factor conditions, including angiogenesis assays, signal pathway and growth factor-related studies, 3D cell culture and differentiation, cell morphology, biochemical function, migration and invasion assays, gene expression studies, tumor cell invasion models, and in vivo tumor xenograft growth support. |
| Principle / Technology | Soluble basement membrane preparation extracted from EHS (Engelbreth-Holm-Swarm) mouse tumor; polymerizes at room temperature to form a biologically active 3D matrix mimicking the in vivo basement membrane. |
| Performance Range / Specifications | Protein concentration: 8-20 mg/mL (specification range); 8-12 mg/mL per product description Endotoxin: <8 EU/mL Free of LDEV (lactate dehydrogenase-elevating virus) No bacterial or fungal growth (strict dialysis and sterilization) Mycoplasma-free (verified by testing) Monthly production capacity above the 50 L scale |
| Reaction Conditions / Protocol | Thaw on ice and keep at 2-8 °C during handling; the matrix polymerizes at room temperature to form a 3D gel. Avoid repeated freeze-thaw cycles. |
| Components / Formulation | Laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), nidogen (entactin), and reduced levels of growth factors including TGF-beta, EGF, IGF, and FGF. Phenol red-free; sterile. |
| Storage Conditions | Store at -20 °C; storage at -80 °C is also acceptable. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 1.5 mL 5 mL 10 mL |
| Product Form | Sterile liquid solution (gels at room temperature). |
| Quality Control | Sterility: no bacterial or fungal growth after strict dialysis and sterilization Endotoxin: <8 EU/mL LDEV: not detected (LDEV-free) Mycoplasma: not detected |
| Concentration | 8-12 mg/mL (per product description); specification range 8-20 mg/mL |
| Source / Origin | Extracted from EHS (Engelbreth-Holm-Swarm) mouse tumor rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C; -80 °C storage is also acceptable. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic or therapeutic procedures. |
| Batch-to-Batch Consistency | Large-scale production with a monthly output above the 50 L scale supports consistent batch supply. |
For research use only, not for clinical use.
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