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| Product Name | Basement Membrane Matrix, GFR, LDEV-Free |
| Catalog No. | RESK-0236 |
| Description | This growth factor reduced (GFR) basement membrane matrix is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin, together with reduced levels of growth factors such as TGF-beta, EGF, IGF, and FGF. The special growth factor reduction process makes it ideal for experiments that require low growth factor conditions, such as studies related to signaling pathways and growth factor signaling. At room temperature, the matrix polymerizes to form a biologically active three-dimensional matrix that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, supporting in vitro cell culture and differentiation. The product is sterile and supplied at a protein concentration of 8-12 mg/mL. |
| Intended Use | For research use in in vitro cell culture and differentiation; studies of cell morphology, biochemical function, migration, invasion, and gene expression. Especially suited for angiogenesis assays and other experiments with stringent requirements on low growth factor levels, including signaling pathway and growth factor-related studies. |
| Principle / Technology | At room temperature the preparation polymerizes into a biologically active three-dimensional matrix that mimics the in vivo basement membrane. The resulting 3D culture substrate promotes the attachment and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells, and can modulate gene expression in 3D cultures of adult rat hepatocytes and human mammary epithelial cells. It also serves as a basic scaffold for tumor cell invasion studies, an essential matrix for in vitro and in vivo angiogenesis research, a 3D scaffold for transplanted tumor cell growth in animal models, and supports peripheral nerve regeneration and differentiation of bovine oviduct epithelial cells. |
| Performance Range / Specifications | Sterile: rigorously dialyzed and sterilized; no bacterial or fungal growth Protein concentration: 8-20 mg/mL Endotoxin: <8 EU/mL LDEV-free (no lactate dehydrogenase-elevating virus residue) Mycoplasma-free (verified by testing) |
| Reaction Conditions / Protocol | Polymerizes at room temperature to form a biologically active three-dimensional matrix. Handle according to standard basement membrane matrix coating and 3D culture procedures. |
| Components / Formulation | Soluble basement membrane preparation extracted from EHS mouse tumor. Main components: laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin; contains reduced levels of growth factors such as TGF-beta, EGF, IGF, and FGF. |
| Storage Conditions | Store at -20 °C. Storage at -80 °C is also acceptable. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 5 mL 10 mL |
| Product Form | Liquid (sterile solution; gels upon polymerization at room temperature) |
| Quality Control | Sterility (no bacterial or fungal growth), protein concentration, endotoxin (<8 EU/mL), LDEV-free, and mycoplasma-free testing are performed. Monthly production capacity exceeds 50 L to ensure stable supply. |
| Concentration | 8-12 mg/mL (lot-specific protein concentration range: 8-20 mg/mL) |
| Source / Origin | EHS (Engelbreth-Holm-Swarm) mouse tumor rich in extracellular matrix proteins |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C; may also be stored at -80 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for angiogenesis assays, signaling pathway studies, and other experiments requiring low growth factor conditions. |
| Application Notes / Precautions | Recommended for applications with stringent growth factor requirements, such as angiogenesis assays. LDEV (lactate dehydrogenase-elevating virus) is an RNA virus that infects mouse macrophages and is a common contaminant of biological materials derived from transplantable tumor cell lines; this product is verified to be free of LDEV. |
| Batch-to-Batch Consistency | Large-scale production (monthly output at the 50 L level or above) supports consistent supply. |
For research use only, not for clinical use.
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