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| Product Name | Basement Membrane Matrix for Organoid Culture (Phenol Red-Free, LDEV-Free Plus) |
| Catalog No. | RESK-0218 |
| Description | This product is a soluble basement membrane preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors, which are rich in extracellular matrix proteins. Its main components are laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin/nidogen, together with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors naturally present in EHS tumors. At room temperature, it polymerizes to form a biologically active three-dimensional matrix that mimics the structure, composition, physical properties, and function of the in vivo basement membrane, supporting the culture and differentiation of cells in vitro and enabling studies of cell morphology, biochemical function, migration, invasion, and gene expression. This phenol red-free, LDEV-free Plus version is an upgraded formulation with improved performance in organoid culture. |
| Intended Use | For research use in organoid culture and three-dimensional cell culture; suitable for studies of cell morphology, biochemical function, migration, invasion, and gene expression. |
| Principle / Technology | Soluble basement membrane preparation extracted from EHS mouse tumor. At room temperature, the matrix polymerizes into a biologically active three-dimensional gel that recapitulates the structure, composition, physical properties, and function of the native basement membrane. |
| Sample Type | Cultured cells, including organoid cultures and 3D cell culture systems. |
| Performance Range / Specifications | Sterility: strict dialysis-based sterilization ensures no bacterial or fungal growth. Protein concentration: 8–20 mg/mL. Endotoxin: <8 EU/mL. Safety: free of LDEV (lactate dehydrogenase-elevating virus). Mycoplasma: verified free of mycoplasma contamination. Production capacity: monthly batch output above 50 L. |
| Components / Formulation | Soluble basement membrane preparation composed mainly of laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and entactin/nidogen, and containing growth factors such as TGF-beta, EGF, IGF, FGF, and tissue plasminogen activator. Phenol red-free; free of LDEV. |
| Storage Conditions | Store at -20 °C. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 5 mL 10 mL 1.5 mL |
| Product Form | Liquid; polymerizes into a three-dimensional gel at room temperature. |
| Quality Control | Each lot is verified for the absence of bacterial and fungal growth (dialysis-based sterilization), protein concentration (8–20 mg/mL), endotoxin (<8 EU/mL), absence of LDEV, and absence of mycoplasma contamination. |
| Purity | Endotoxin <8 EU/mL; free of LDEV; verified free of mycoplasma, bacteria, and fungi. |
| Concentration | Protein concentration 8–20 mg/mL |
| Source / Origin | Engelbreth-Holm-Swarm (EHS) mouse tumor |
| Shipping Conditions | Shipped on dry ice. |
| Application Notes / Precautions | The matrix polymerizes at room temperature to form a three-dimensional gel; keep the product cold during handling to avoid premature gelation. Phenol red-free formulation avoids interference with fluorescence-based assays and hormone-sensitive studies. |
| Batch-to-Batch Consistency | Large-scale production with monthly batch output above 50 L supports lot-to-lot consistency and stable supply. |
For research use only, not for clinical use.
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