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| Product Name | Basement Membrane Matrix for Organoid Culture (Phenol Red-Free, LDEV-Free) |
| Catalog No. | RESK-0224 |
| Description | This basement membrane matrix for organoid culture is a soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components include laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), and nidogen (entactin), together with growth factors such as TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors naturally present in the EHS tumor. At room temperature, the matrix polymerizes to form a biologically active three-dimensional scaffold that mimics the structure, composition, physical properties, and function of the in vivo cell basement membrane, supporting in vitro cell culture and differentiation and enabling studies of cell morphology, biochemical function, migration, invasion, and gene expression. The product is sterile, phenol red-free, and LDEV-free, and has been validated for organoid culture, meeting the requirements of organoid construction. Organoids of common cancer types can be cultured with this matrix; demonstrated applications include the culture of pancreatic cancer, cervical cancer, and mouse small intestinal organoids. |
| Intended Use | For the establishment and culture of organoids, including organoids of common cancer types (e.g., pancreatic cancer, cervical cancer) and mouse small intestinal organoids; also suitable for 3D cell culture and studies of cell morphology, biochemical function, migration, invasion, and gene expression. |
| Principle / Technology | Soluble basement membrane preparation extracted from EHS (Engelbreth-Holm-Swarm) mouse tumor; polymerizes at room temperature to form a biologically active 3D matrix mimicking the in vivo basement membrane; validated for organoid culture. |
| Performance Range / Specifications | Protein concentration: 8-20 mg/mL Endotoxin: <8 EU/mL Free of LDEV (lactate dehydrogenase-elevating virus) No bacterial or fungal growth (strict dialysis and sterilization) Mycoplasma-free (verified by testing) Monthly batch production capacity above the 50 L scale |
| Reaction Conditions / Protocol | Thaw on ice and keep at 2-8 °C during handling; the matrix polymerizes at room temperature to form a 3D gel suitable for organoid embedding and culture. Avoid repeated freeze-thaw cycles. |
| Components / Formulation | Laminin, collagen type IV, heparan sulfate proteoglycan (HSPG), nidogen (entactin), and growth factors including TGF-beta, EGF, IGF, FGF, tissue plasminogen activator, and other growth factors naturally present in EHS tumor. Phenol red-free; sterile. |
| Storage Conditions | Store at -20 °C. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 2 years when stored at -20 °C. |
| Package Specifications | 5 mL 10 mL |
| Product Form | Sterile liquid solution (gels at room temperature). |
| Quality Control | Sterility: no bacterial or fungal growth after strict dialysis and sterilization Endotoxin: <8 EU/mL LDEV: not detected (LDEV-free) Mycoplasma: not detected Functionally validated for organoid culture (pancreatic cancer, cervical cancer, and mouse small intestinal organoids) |
| Concentration | 8-20 mg/mL |
| Source / Origin | Extracted from EHS (Engelbreth-Holm-Swarm) mouse tumor rich in extracellular matrix proteins. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 2 years at -20 °C. |
| Regulatory / Compliance | For research use only. Not for use in diagnostic or therapeutic procedures. |
| Batch-to-Batch Consistency | Large-scale production with a monthly batch output above the 50 L scale supports consistent batch supply. |
For research use only, not for clinical use.
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