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| Product Name | BamHI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0388 |
| Description | A workhorse for gene cloning and vector construction, this rapid recombinant endonuclease cuts 5 prime G^GATCC 3 prime and finishes digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. The preparation is standardised at 20 U/uL and runs in the shared rapid series buffer at its 37°C optimum. Dam, Dcm, CpG, EcoKI and EcoBI methylation have no effect on activity, and no isoschizomers with alternative names are documented. Note that this enzyme cannot be heat inactivated; after digestion, remove it by phenol chloroform extraction or column purification. |
| Intended Use | Gene cloning and vector construction with rapid digestion of plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime G^GATCC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 T |
| Key Features | One shared buffer across the series; high activity redundancy; large 500 reaction pack for routine cloning |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Restriction digest pattern on agarose gel |
For in vitro diagnostic use.
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