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| Product Name | B-27 Supplement, 50x, Serum-Free Neuronal Supplement |
| Catalog No. | ATR-CCR-0022 |
| Description | A 50-fold concentrated serum-free supplement containing optimized concentrations of antioxidants, hormones, and vitamins for the long-term culture and maintenance of neurons and neural stem cells. |
| Intended Use | Supplementing Neurobasal medium for the culture of primary hippocampal and cortical neurons, neural stem cells, embryonic stem cell-derived neural lineages, and iPSC-derived neurons. |
| Principle / Technology | Formulation includes antioxidants (catalase, superoxide dismutase, glutathione), hormones (corticosterone, progesterone, triiodothyronine), vitamins (biotin, B12), and essential fatty acids (linoleic, linolenic) that support neuronal survival and inhibit glial proliferation. |
| Detection Method | Neuronal viability by NeuN immunostaining, neurite outgrowth measurement, electrophysiological activity by patch clamp |
| Sample Type | Primary rat and mouse hippocampal neurons, cortical neurons, neural stem cells, ES/iPSC-derived neural progenitors |
| Performance Range / Specifications | Maintains neuronal cultures >4 weeks; glial cell proportion <5% in primary neuronal cultures |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 1 mL B-27 supplement per 50 mL Neurobasal medium (2% v/v final); prepare complete medium fresh; use within 2 weeks; protect from light. |
| Components / Formulation | Biotin, BSA, Catalase, Corticosterone, Ethanolamine, Fetuin, Galactose, Glutathione (reduced), Insulin, Linoleic Acid, Linolenic Acid, Progesterone, Putrescine, Retinyl Acetate, Selenium, Superoxide Dismutase, T3 (triiodothyronine), DL-Alpha-Tocopherol, DL-Alpha-Tocopherol Acetate, Transferrin |
| Storage Conditions | -20°C (long term); 2–8°C after thawing, protect from light |
| Shelf Life | 24 months at -20°C; 2 weeks at 2–8°C in complete medium |
| Package Specifications | 10 mL, 50 mL |
| Product Form | Liquid concentrate (50x), sterile |
| Quality Control | Neuronal viability and neurite outgrowth assay, sterility, endotoxin, lot-to-lot consistency testing |
| Key Features | Serum-free; inhibits glial proliferation; supports long-term neuronal culture; validated for primary neurons and stem cell-derived neurons; antioxidant rich |
For research use only, not for clinical use.
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