AvrII Rapid Restriction Endonuclease (5 U/uL)
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AvrII Rapid Restriction Endonuclease (5 U/uL)

Cat.No: IVDRM-0374 Datasheet

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Product Name AvrII Rapid Restriction Endonuclease (5 U/uL)
Catalog No. IVDRM-0374
Description This recombinant rapid endonuclease recognises 5 prime C^CTAGG 3 prime and delivers precise cleavage of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. Supplied at 5 U/uL, it runs in the universal rapid digestion buffer shared by the whole series, keeping reaction assembly simple, and its activity redundancy absorbs substrate overload and difficult templates. Incubation at 80°C for 20 minutes inactivates the enzyme after digestion. Dam, Dcm, CpG, EcoKI and EcoBI methylation do not affect cutting, and the site is shared by the isoschizomers AspA2I, BlnI and XmaJI.
Intended Use Fast and precise cutting of plasmid DNA, PCR products and genomic DNA
Principle / Technology Site specific cleavage at 5 prime C^CTAGG 3 prime within 5 to 15 minutes at 37°C
Specificity Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers AspA2I, BlnI and XmaJI
Reaction Conditions / Protocol Digest in 1x rapid restriction buffer at 37°C; heat inactivate at 80°C for 20 minutes
Storage Conditions -25 to -15°C
Shelf Life 2 years
Package Specifications 25 T
Key Features One shared buffer for the entire rapid series; tolerant of substrate excess and challenging templates
Concentration 5 U/uL
Readouts / Downstream Assays Agarose gel analysis of restriction fragments

For in vitro diagnostic use.

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