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| Product Name | AvaII Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0383 |
| Description | Targeting the degenerate sequence 5 prime G^GWCC 3 prime, where W is A or T, this rapid recombinant endonuclease handles plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 10 U/uL, it digests in the common rapid series buffer at 37°C and is inactivated by 20 minutes at 80°C CpG and Dcm methylation overlapping the site may impede cutting, an point worth noting for methylated substrates, and Bme18I and Eco47I are recognised isoschizomers. Downstream genomic DNA processing for analytical workflows is a typical application. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for downstream analysis |
| Principle / Technology | Site specific cleavage at 5 prime G^GWCC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG and Dcm methylation may block cleavage; isoschizomers Bme18I and Eco47I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | One buffer shared across the series; reliable on excess or difficult templates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of restriction fragments |
For in vitro diagnostic use.
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