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| Product Name | AseI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0381 |
| Description | Recognising 5 prime ATTA^AT 3 prime, this engineered rapid endonuclease produces clean cuts in plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. The preparation is standardised at 10 U/uL and digests in the common series buffer at 37°C, with inactivation afterwards at 80°C for 20 minutes. EcoBI methylation influences cleavage at overlapping sequences, and PshBI and VspI are established isoschizomers of this enzyme. |
| Intended Use | Rapid preparation of DNA fragments from plasmid DNA, PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime ATTA^AT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation affects cleavage; isoschizomers PshBI and VspI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Common buffer shared by the entire rapid series; activity margin for excess substrate and difficult templates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Gel electrophoresis profile of digested DNA |
For in vitro diagnostic use.
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