- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | AscI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0373 |
| Description | Generated through genetic engineering recombinant technology, this rapid endonuclease completes precise DNA cleavage within 5 to 15 minutes and suits fast digestion of plasmid DNA, PCR products and genomic DNA. It recognises the palindromic site 5 prime GG^CGCGCC 3 prime and is supplied at 10 U/uL. All enzymes in this rapid series share one digestion buffer, which simplifies reaction setup, and generous activity redundancy lets the enzyme handle substrate excess or difficult templates with ease. Heat inactivation is achieved by incubating at 80°C for 20 minutes, and the isoschizomers PalAI and SgsI cut the same site. CpG methylation fully overlapping the site blocks cleavage while Dam, Dcm, EcoKI and EcoBI methylation have no effect. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning |
| Principle / Technology | Site specific cleavage at 5 prime GG^CGCGCC 3 prime with 5 to 15 minute digestion in a shared rapid buffer |
| Specificity | CpG methylation fully overlapping the recognition site blocks cleavage; Dam, Dcm, EcoKI and EcoBI methylation have no effect; isoschizomers PalAI and SgsI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single shared buffer across the rapid series; high activity redundancy for excess substrate or difficult templates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel pattern of digested DNA |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
