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| Product Name | ArCas12a Nuclease for CRISPR Editing and Nucleic Acid Detection (10 uM) |
| Catalog No. | IVDRM-0417 |
| Description | Derived from the CRISPR system of Agathobacter rectalis and also known as Cpf1, ArCas12a is a 1263 amino acid monomeric class 2 type V effector that functions without a trans activating crRNA. Guided by crRNA alone through its single RuvC domain, it recognises thymine rich PAM motifs at the 5 prime end of the target and cuts double stranded DNA to leave sticky ends, and it also processes its own CRISPR RNA. Once activated it additionally exhibits trans cleavage, indiscriminately shredding non target single stranded DNA in the reaction, a property widely harnessed for CRISPR based nucleic acid detection. Compared with LbCas12a and AsCas12a it tolerates a broader temperature span of 25 to 55°C, easing assay development. The preparation is verified free of exonuclease and RNase residues. |
| Intended Use | Genome editing in mammalian and plant systems and CRISPR based nucleic acid detection using trans cleavage activity |
| Principle / Technology | crRNA guided RuvC domain cleavage at thymine rich PAM motifs with collateral single stranded DNA trans cleavage upon activation |
| Detection Method | Double stranded DNA targets with T rich PAM; non target ssDNA via trans cleavage |
| Performance Range / Specifications | Cis and trans cleavage activity verified; temperature tolerance 25 to 55°C |
| Specificity | Class 2 type V CRISPR effector with RuvC domain only; sticky end formation; no exonuclease or RNase residues |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 pmol; 1000 pmol |
| Key Features | No trans activating crRNA required; broader temperature range than LbCas12a and AsCas12a |
| Concentration | 10 uM |
| Source / Origin | Agathobacter rectalis, monomeric protein of 1263 amino acids |
| Readouts / Downstream Assays | In vitro dsDNA cleavage assay; fluorescent ssDNA reporter assay |
For in vitro diagnostic use.
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