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| Product Name | ApeKI Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0523 |
| Description | From the hyperthermophilic aerobic archaeon Aeropyrum pernix K1, ApeKI is a type IIP restriction enzyme that recognises and cuts 5 prime G^CWGC 3 prime to leave a three base sticky overhang. CpG methylation overlapping the site blocks cleavage. The enzyme performs at 75°C in its dedicated Cut Buffer C and is not heat inactivatable, so the enzyme is removed after digestion by phenol chloroform extraction or column purification. As one of the first-choice enzymes for genotyping-by-sequencing, it is widely used in plant and animal genetic research, population evolution analysis and functional gene localisation. |
| Intended Use | Genotyping-by-sequencing library construction, genetic marker development, population evolution analysis and gene localisation |
| Principle / Technology | Recognition and cleavage of 5 prime G^CWGC 3 prime to a three base sticky overhang at 75°C |
| Sample Type | Genomic DNA for reduced representation library construction |
| Specificity | CpG methylation overlapping the site blocks cleavage; three base sticky overhang |
| Reaction Conditions / Protocol | Digest in 1x Cut Buffer C at 75°C; not heat inactivatable, remove enzyme by phenol chloroform extraction or column purification |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 U |
| Key Features | First-choice GBS enzyme; hyperthermophile derived with a 75°C working temperature |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel profile of digested genomic DNA |
For in vitro diagnostic use.
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