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| Product Name | Antimicrobial Agent for Primary Cells |
| Catalog No. | RESK-0520 |
| Description | Antimicrobial Agent for Primary Cells is a sterile-filtered, pale yellow solution intended for primary cell culture. The stock concentration is 50 mg/mL, and the recommended working concentration is 100-300 ug/mL. It is a broad-spectrum antibiotic formulation designed to protect primary cells from microbial contamination. Primary cell models are highly susceptible to contamination by natural flora from the host animal or from the cell isolation process, and this product provides comprehensive protection against contaminants, including Gram-positive bacteria (e.g., Bacillus and Staphylococcus species), Gram-negative bacteria (e.g., Escherichia coli, Enterobacter, Pseudomonas aeruginosa, and Acinetobacter), mycoplasma, and fungi. It provides maximal protection of primary cells with minimal cytotoxicity when acting against microbial contamination. The formulation consists of multiple antibiotics: three of them act on mycoplasma, Gram-positive, and Gram-negative bacteria by targeting DNA gyrase and the prokaryotic ribosomal subunits (30S and 50S), thereby blocking DNA synthesis and protein synthesis respectively, while additional components eradicate fungi, including yeasts. |
| Intended Use | For the protection of primary cell cultures from microbial contamination. Also suitable for organoid culture and stem cell culture, and can be used throughout the entire cell culture process (e.g., in culture media and wash solutions). |
| Principle / Technology | A combination of multiple antibiotics: three components act on mycoplasma and on Gram-positive and Gram-negative bacteria by targeting DNA gyrase and the prokaryotic ribosomal subunits (30S and 50S), thereby blocking DNA synthesis and protein synthesis respectively; additional components eradicate fungi, including yeasts. |
| Detection Method | Antibacterial efficacy was evaluated by in vitro microbial growth inhibition (inhibition zone) assays; cytotoxicity was assessed by CCK-8 assay and cell counting. |
| Performance Range / Specifications | Broad-spectrum protection: prevents contamination by bacteria, fungi (including yeasts), and mycoplasma. Strong versatility: suitable for primary cell culture, organoid culture, and stem cell culture. Multiple application scenarios: can be used throughout the cell culture process, including in culture media and wash solutions. Low cytotoxicity: minimal toxicity to cells, maximally preserving the viability and normal growth of primary cells. |
| Specificity | Active against Gram-positive bacteria (e.g., Bacillus and Staphylococcus species), Gram-negative bacteria (e.g., Escherichia coli, Enterobacter, Pseudomonas aeruginosa, and Acinetobacter), mycoplasma, and fungi, including yeasts. |
| Reaction Conditions / Protocol | Add to culture medium or wash solutions at a recommended working concentration of 100-300 ug/mL (diluted from the 50 mg/mL stock). Can be used throughout the entire cell culture process. |
| Components / Formulation | A mixture of multiple antibiotics: three components target mycoplasma, Gram-positive, and Gram-negative bacteria by acting on DNA gyrase and the prokaryotic ribosomal subunits (30S and 50S); additional components eradicate fungi, including yeasts. Supplied as a sterile-filtered, pale yellow solution. |
| Storage Conditions | Store at -25 to -15 °C upon receipt and avoid repeated freeze-thaw cycles. Alternatively, store at 2-8 °C for short-term storage. |
| Shelf Life | 12 months at -25 to -15 °C; 1 month at 2-8 °C. |
| Package Specifications | 1 mL 5 mL 10 mL |
| Product Form | Sterile-filtered, pale yellow liquid solution. |
| Quality Control | In inhibition zone assays performed at working concentrations of 33, 50, and 100 ug/mL against bacterial suspensions, this product showed stronger bacterial growth inhibition than a comparable market product. Cytotoxicity testing by CCK-8 assay and cell counting showed virtually no effect on cell viability or proliferation after treatment. |
| Concentration | Stock concentration: 50 mg/mL. Recommended working concentration: 100-300 ug/mL. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 12 months when stored at -25 to -15 °C with avoidance of repeated freeze-thaw cycles; alternatively, valid for 1 month when stored at 2-8 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for primary cell culture, organoid culture, and stem cell culture; can be used throughout the cell culture process, including in culture media and wash solutions. |
For research use only, not for clinical use.
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