Anti-Rabbit Tn5 Transposase-Nanobody Fusion Enzyme (bare enzyme)

Name: Anti-Rabbit Tn5 Transposase-Nanobody Fusion Enzyme (bare enzyme)
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Cat.No: IVDRM-0324 Datasheet

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Product Name	Anti-Rabbit Tn5 Transposase-Nanobody Fusion Enzyme (bare enzyme)
Catalog No.	IVDRM-0324
Description	A fusion protein joining a rabbit-specific nanobody to Tn5 transposase, built for CUT&Tag experiments that use rabbit primary antibodies. Supplied as a bare enzyme without pre-loaded adapters, users assemble their own adapter sequences, and universal adapter loading can be customised on request. The enzyme supports single-target and multi-target CUT&Tag library construction across histone marks including H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K9ac.
Intended Use	CUT&Tag and multi-target CUT&Tag library construction with rabbit primary antibodies; histone modification and chromatin protein-DNA interaction profiling.
Principle / Technology	The nanobody binds rabbit primary antibody on chromatin while the fused Tn5 transposase cuts and tags adjacent DNA with assembled adapters.
Sample Type	Chromatin preparations from cultured cells or tissue
Performance Range / Specifications	Compatible histone marks include H3K4me1, H3K4me3, H3K9me3, H3K27ac, H3K27me3 and H3K9ac.
Specificity	Rabbit nanobody specificity; bare-enzyme format for custom adapter design.
Reaction Conditions / Protocol	Incubate with primary-antibody-bound chromatin, then activate transposition after adapter assembly.
Components / Formulation	Anti-rabbit nanobody-Tn5 fusion enzyme.
Storage Conditions	-85 to -65 °C
Package Specifications	4 T, 12 T, 48 T
Product Form	Bare enzyme solution
Key Features	Custom adapter flexibility; customisable universal adapter loading; single-target and multi-target workflows.
Source / Origin	Recombinant nanobody-Tn5 fusion protein
Readouts / Downstream Assays	CUT&Tag chromatin profiling libraries

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For in vitro diagnostic use.