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| Product Name | Antarctic Phosphatase, Heat-Labile Alkaline Phosphatase (5 U/uL) |
| Catalog No. | IVDRM-0578 |
| Description | A recombinant heat-labile alkaline phosphatase from an Antarctic bacterium that occurs as both homodimer and monomer, Antarctic Phosphatase removes 5 prime and 3 prime phosphate monoesters from DNA, RNA and proteins non-specifically and also hydrolyses ribonucleoside and deoxyribonucleoside triphosphates. It acts on 5 prime and 3 prime overhangs and on blunt ends and stays active in a range of restriction and PCR buffers. Because 5 minutes at 75°C inactivates it completely and irreversibly, no removal step is needed before ligation or end labelling. Beyond nucleic acid work the enzyme also dephosphorylates serine, threonine and tyrosine residues on proteins. |
| Intended Use | Vector dephosphorylation to lower self-ligation, nucleic acid end processing and protein dephosphorylation |
| Principle / Technology | Non-specific hydrolysis of 5 prime and 3 prime phosphate monoesters on DNA, RNA, proteins, NTPs and dNTPs |
| Sample Type | DNA and RNA ends, NTP and dNTP pools, and phosphorylated proteins |
| Specificity | Acts on 5 prime and 3 prime overhangs and blunt ends; low nickase, exonuclease, DNase and RNase residue |
| Reaction Conditions / Protocol | Incubate at 37°C for 15 minutes to complete dephosphorylation and heat at 75°C for 5 minutes for irreversible inactivation |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5000 U |
| Key Features | Irreversible heat inactivation at 75°C; compatible with many restriction and PCR buffers |
| Purity | Low residual nickase, exonuclease, DNase and RNase |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Ligation efficiency and colony PCR after vector dephosphorylation |
For in vitro diagnostic use.
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