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| Product Name | Angiotensin Converting Enzyme (ACE) Activity Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0032 |
| Description | Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxyl peptidase, mainly existing in endothelial cells of various tissues such as the lungs, brain, and kidneys. Its main function is to inactivate bradykinin and catalyze the conversion of angiotensin I to angiotensin II, playing an important regulatory role in the angiotensin system. And it is closely related to physiological processes such as blood pressure, cardiovascular function and water-salt balance. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well UV plate |
| Number of Testable Samples | 96 Samples |
| Estimated Measurement Time | 4 h (96 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Angiotensin-converting enzyme catalyzes the hydrolysis of the substrate N-[3-(2-furyl)acryloyl]-L-phenylalanyl-glycyl-glycyl-glycine (FAPGG) to produce furanacryloyl-L-phenylalanine (FAP) and bis-glycyl-glycyl-glycine peptide (GG), with a characteristic peak at 340 nm, which is characterized by a change in absorbance value. |
| Detection Methods | FAPGG Method |
| Detection Wavelength | 340 nm |
| Signal Response | Decremental |
| Note | Accurately complete the determination of absorbance values at the specified time points to ensure the accuracy and repeatability of the experimental results. If an microplate reader is used, multi-channel pipettes should be employed and the tests should be conducted in batches to ensure consistent reaction times between groups. If the A1 determination is greater than 1.0 or the ΔA determination is greater than 0.3, it is recommended to appropriately dilute the crude enzyme solution using reagent One before conducting the determination. If the determination of ΔA is less than 0.02, it is recommended to conduct the determination after appropriately increasing the enzymatic reaction time or the sample size, and make corresponding modifications during the calculation. |
For research use only, not for clinical use.
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