| Product Name | Direct Bilirubin (DBIL) Content Assay Kit (Microplate Reader) |
| Catalog No. | BBITK-HMM-0028 |
| Description | Direct bilirubin (DBIL), also known as conjugated bilirubin, is formed when indirect bilirubin enters the liver and combines with glucuronic acid under the action of glucuronosyltransferase in the liver. An increase in direct bilirubin is of great significance for the clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholestasis, cholangiocarcinoma, etc. |
| Testing Equipment | Microplate Reader |
| Matching | 96-well plate |
| Number of Testable Samples | 96 Samples |
| Estimated Measurement Time | 3 h (96 Samples) |
| Storage | Store at 4°C |
| Self-contained Reagents | / |
| Detection Principle | Direct bilirubin can be oxidized by sodium nitrite to form biliverdin, bilirubin has a characteristic absorption peak at 450 nm, and the change of absorbance value can be used to quantify the content of direct bilirubin. |
| Detection Methods | Sodium Nitrite Oxidation Method |
| Detection Wavelength | 450 nm |
| Signal Response | Decremental |
| Note | Bilirubin is easily decomposed in the presence of light, the operation should be carried out under the condition of avoiding light; if ΔA is greater than 0.5, it is recommended to dilute the sample to be measured appropriately before measurement, and the calculation should be modified accordingly. |
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