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| Product Name | Amphotericin B (Fungizone), 250 µg/mL, 100x Solution |
| Catalog No. | ATR-CCR-0014 |
| Description | A sterile 100-fold concentrated Amphotericin B solution for the prevention of fungal and yeast contamination in mammalian cell culture, formulated as a solubilized preparation in deoxycholate. |
| Intended Use | Antifungal prophylaxis in cell culture media to prevent contamination by Candida, Aspergillus, and Saccharomyces species during extended culture experiments and primary cell isolation procedures. |
| Principle / Technology | Amphotericin B binds to ergosterol in fungal cell membranes, forming transmembrane channels that increase permeability to monovalent ions, leading to cell death; mammalian cells are relatively resistant due to cholesterol versus ergosterol in their membranes. |
| Detection Method | Antifungal efficacy with Candida albicans challenge, cell cytotoxicity at working concentration |
| Sample Type | Mammalian cell culture media requiring antifungal protection, particularly for primary cell isolation from potentially contaminated tissue sources |
| Performance Range / Specifications | Working concentration 2.5 µg/mL at 1:100 dilution; effective against Candida, Aspergillus, and most other fungi |
| Sensitivity / LOD | N/A |
| Specificity | Antifungal; limited antibacterial activity; ineffective against viruses and mycoplasma |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution); use at working concentration continuously; some cell lines may show sensitivity at concentrations above 2.5 µg/mL. |
| Components / Formulation | Amphotericin B 250 µg/mL, sodium deoxycholate as solubilizing agent, in sterile water |
| Storage Conditions | -20°C (long term); 2–8°C after thawing, protect from light; do not refreeze |
| Shelf Life | 12 months at -20°C; 4 weeks at 2–8°C after thawing |
| Package Specifications | 50 mL, 100 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Potency by antimicrobial assay, sterility, endotoxin, cytotoxicity validation with reference cell lines |
| Key Features | Sterile 100x concentrate; effective against broad spectrum of fungi; validated for mammalian cell culture; light sensitive; compatible with penicillin-streptomycin |
For research use only, not for clinical use.
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