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| Product Name | AgeI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0376 |
| Description | Recognising 5 prime A^CCGGT 3 prime, this engineered rapid endonuclease linearises plasmids and cuts PCR products or genomic DNA within 5 to 15 minutes. The preparation is standardised at 20 U/uL and performs in the common rapid series buffer at 37°C, with heat inactivation at 80°C for 20 minutes afterwards. CpG methylation at the overlapping sequence obstructs cleavage, and the same site is recognised by the isoschizomers AsiGI, CspAI, BshTI and PinAI. Applications span plasmid linearisation, vector construction and general DNA fragmentation. |
| Intended Use | Plasmid linearisation, vector construction and rapid fragmentation of PCR products and genomic DNA |
| Principle / Technology | Site specific cleavage at 5 prime A^CCGGT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; isoschizomers AsiGI, CspAI, BshTI and PinAI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T; 200 T |
| Key Features | One buffer shared across the rapid series; comfortable activity margin for excess substrate |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion pattern on agarose gel |
For in vitro diagnostic use.
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