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| Product Name | AflII Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0379 |
| Description | With specificity for 5 prime C^TTAAG 3 prime, this recombinant rapid endonuclease prepares DNA fragments for cloning and gene engineering work, cutting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. The enzyme ships at 20 U/uL and performs in the series wide shared buffer at 37°C, with heat inactivation at 80°C for 20 minutes. Dam, Dcm, CpG, EcoKI and EcoBI methylation have no influence on activity, and BspTI, BfrI, BstAFI, MspCI and Vha464I are recognised isoschizomers. |
| Intended Use | DNA fragment preparation and cloning for molecular biology and gene engineering applications |
| Principle / Technology | Site specific cleavage at 5 prime C^TTAAG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers BspTI, BfrI, BstAFI, MspCI and Vha464I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | One shared buffer for the series; reliable digestion of excess or difficult substrates |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of digested DNA |
For in vitro diagnostic use.
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