- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | AfeI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0386 |
| Description | Cutting the palindromic site 5 prime AGC^GCT 3 prime to yield blunt ended fragments, this rapid recombinant endonuclease supports DNA fragment preparation, vector construction and digest based plasmid verification. It completes digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 10 U/uL, and runs in the common rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation overlapping the site blocks cleavage, and Eco47III is the recognised isoschizomer. |
| Intended Use | DNA fragment preparation, vector construction and plasmid verification by diagnostic digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime AGC^GCT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; isoschizomer Eco47III |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 20 T |
| Key Features | Blunt end output simplifies downstream ligation design; single shared buffer across the series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of digest products |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
