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| Product Name | Advanced Reverse Transcriptase, Ultra-Low Residual (200 U/uL) |
| Catalog No. | IVDRM-0517 |
| Description | Obtained from M-MLV (H-) Reverse Transcriptase through genetic engineering, this reverse transcriptase shows greatly improved reaction speed and stability compared with the parent enzyme. A dedicated refining process keeps nuclease, host genomic DNA and background bacterial genomic DNA residue extremely low, with E. coli genomic DNA below 0.02 copies per 100 U, which suits development of rapid fully pre-mixed RT-qPCR reagents and use as an enzyme raw material for IVD molecular diagnostics. A glycerol-free version is available on request. |
| Intended Use | cDNA synthesis for RT-PCR and RT-qPCR, and enzyme raw material for IVD molecular diagnostic assay development |
| Principle / Technology | RNA directed DNA polymerase activity engineered from M-MLV (H-) reverse transcriptase with improved speed and stability |
| Sample Type | RNA templates for reverse transcription |
| Reaction Conditions / Protocol | Combine with RNA template and primers for reverse transcription ahead of PCR or qPCR detection |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 1 year |
| Package Specifications | 10 KU; 100 KU; 200 KU |
| Key Features | Greatly improved speed and stability over the parent enzyme; a glycerol-free version is available |
| Purity | E. coli genomic DNA below 0.02 copies per 100 U; nuclease and background bacterial genomic DNA residue extremely low |
| Concentration | 200 U/uL |
| Readouts / Downstream Assays | RT-qPCR amplification curves from RNA templates |
For in vitro diagnostic use.
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