Advanced Reverse Transcriptase, Glycerol-Free, Ultra-Low Residual (200 U/uL)

Name: Advanced Reverse Transcriptase, Glycerol-Free, Ultra-Low Residual (200 U/uL)
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Cat.No: IVDRM-0518 Datasheet

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Product Name	Advanced Reverse Transcriptase, Glycerol-Free, Ultra-Low Residual (200 U/uL)
Catalog No.	IVDRM-0518
Description	Engineered from M-MLV (H-) Reverse Transcriptase, this reverse transcriptase reacts faster and more stably than the parent enzyme, and a refining process leaves nuclease, host genomic DNA and background bacterial genomic DNA residue extremely low at under 0.02 copies of E. coli genomic DNA per 100 U. The formulation contains no glycerol, so it can be used to prepare lyophilised RT-qPCR reagents, and lyophilised assays retain high detection sensitivity and good stability. After 5 days of accelerated storage at 37°C the amplification curve shape, fluorescence values and Ct values remain unchanged, and the enzyme also serves library construction for high throughput sequencing.
Intended Use	Lyophilised RT-qPCR reagent development, rapid fully pre-mixed assays and NGS library construction
Principle / Technology	RNA directed DNA polymerase activity engineered from M-MLV (H-) reverse transcriptase in a glycerol-free formulation
Sample Type	RNA templates for reverse transcription
Specificity	Stability unchanged after 5 days of accelerated storage at 37°C
Reaction Conditions / Protocol	Combine with the RNA template for reverse transcription; suitable for lyophilised reaction formulations
Storage Conditions	-25 to -15°C
Shelf Life	1 year
Package Specifications	10 KU; 100 KU; 200 KU
Key Features	Glycerol-free and suited to lyophilised systems; Ct values stable through accelerated storage
Purity	E. coli genomic DNA below 0.02 copies per 100 U; nuclease residue extremely low
Concentration	200 U/uL
Readouts / Downstream Assays	RT-qPCR amplification curves after lyophilisation and storage

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For in vitro diagnostic use.