Advanced Heat-Labile Uracil DNA Glycosylase (UDG/UNG) (10 U/uL)
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Advanced Heat-Labile Uracil DNA Glycosylase (UDG/UNG) (10 U/uL)

Cat.No: IVDRM-0577 Datasheet

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Product Details Related Products
Product Name Advanced Heat-Labile Uracil DNA Glycosylase (UDG/UNG) (10 U/uL)
Catalog No. IVDRM-0577
Description Supplied at 10 U/uL, this advanced format of the heat-labile uracil-DNA glycosylase hydrolyses the N-glycosidic bond between uracil and the sugar-phosphate backbone of DNA strands carrying dU, releasing free uracil, and acts on both single and double stranded DNA without affecting RNA. Manufactured by an ultra-low residual process, it carries only trace nuclease and host gDNA contamination, which suits applications that tolerate the least background, such as quantitative PCR systems in which primers and probes are pre-mixed ahead of use. The high enzyme concentration eases formulation of concentrated master mixes.
Intended Use Contamination control in full pre-mix RT-qPCR systems and other low background molecular assays
Principle / Technology Hydrolysis of the N-glycosidic bond of uracil in DNA with heat-labile behaviour suited to pre-mix systems
Sample Type dU-containing single and double stranded DNA in pre-mixed PCR and RT-qPCR systems
Specificity Acts on dU-containing single and double stranded DNA; heat labile; no activity on RNA
Reaction Conditions / Protocol Include in the master mix before amplification and inactivate by heating before the amplification step
Storage Conditions -25 to -15°C
Shelf Life 2 years
Package Specifications 500 U
Key Features Very low nuclease and host gDNA residue; high concentration suits concentrated pre-mixed master mixes
Concentration 10 U/uL
Readouts / Downstream Assays Carryover control performance in pre-mixed RT-qPCR systems

For in vitro diagnostic use.

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