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| Product Name | Adhesive PCR Plate Sealing Film, Optical Clear, Pressure-Sensitive |
| Catalog No. | IGM-HMM-0066 |
| Description | An optically clear adhesive sealing film specifically designed for PCR and qPCR plate sealing. The pressure-sensitive silicone-based adhesive forms a tight seal over each well rim, preventing evaporation and cross-well contamination during thermal cycling. The film is optically transparent from 260–700 nm, enabling fluorescence reading directly through the seal without removal. The seal is pierceable for sample recovery after PCR. |
| Intended Use | Evaporation-proof sealing of PCR and qPCR microplates with optical transparency for through-seal fluorescence detection. |
| Principle / Technology | Pressure-sensitive silicone adhesive forming individual well seals with optical-grade polyester film backing |
| Detection Method | Fluorescence reading through film on qPCR instruments; end-point PCR product recovery by needle puncture |
| Sample Type | 96-well and 384-well PCR plates for endpoint, real-time, and digital PCR |
| Performance Range / Specifications | Film thickness: 50 µm; optical transmission: ≥90% at 400–700 nm; temperature resistance: –80 °C to +120 °C; sealing temperature: room temperature (pressure-sensitive) |
| Sensitivity / LOD | Evaporation loss ≤0.5% per well after 40 PCR cycles with heated lid at 105 °C |
| Specificity | Non-fluorescent film, DNase/RNase/pyrogen-free; does not autofluoresce in common qPCR detection channels |
| Reaction Conditions / Protocol | Using an applicator tool or squeegee, apply film to dry plate surface; press firmly around each well rim and plate perimeter; apply plate to thermal cycler with heated lid |
| Components / Formulation | Optical clear adhesive sealing film, 50 sheets per pack, interleaved with protective backing |
| Storage Conditions | Room temperature, in sealed packaging |
| Shelf Life | 36 months |
| Package Specifications | 50 sheets or 100 sheets per pack |
| Product Form | Thin film sheet with removable paper backing |
| Quality Control | Optical clarity verified by spectrophotometer; adhesive seal integrity tested by vacuum leak test; PCR-compatible validated by real-time PCR with no inhibition |
| Key Features | Pressure-sensitive adhesive requires no heat sealing equipment, reducing workflow complexity compared to heat-seal foils |
| Purity | Sterile (SAL 10⁻⁶); DNase/RNase free; pyrogen-free; non-cytotoxic |
| Concentration | Not applicable — solid consumable product |
| Activity / Unit Definition | Performance verified per dimensional and functional specifications |
| Molecular Weight | Not applicable — polymer/glass consumable product |
| Source / Origin | Virgin polypropylene, polystyrene, borosilicate glass as specified |
| pH Range / Optimal pH | Not applicable — solid consumable |
| Shipping Conditions | Ambient temperature in protective packaging |
| Expiration Date / Stability | 36 months in sealed packaging; sterility maintained until package opened |
| Regulatory / Compliance | ISO 13485; CE-marked; FDA registered; USP Class VI materials |
| Compatibility | Compatible with standard laboratory pipettes, thermal cyclers, and centrifuges |
| Recommended Buffer System | Not applicable — use with standard aqueous buffers and organic solvents per chemical compatibility |
| Application Notes / Precautions | Single use only for sterile products; inspect packaging integrity before use; dispose per biohazard guidelines |
| Batch-to-Batch Consistency | Dimensional tolerance within specification; functional testing per AQL sampling plan |
For research use only, not for clinical use.
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