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| Product Name | Adhesive PCR Plate Sealing Film, Optical Clear |
| Catalog No. | IGM-HMM-0054 |
| Description | Ultra-clear polyester-based adhesive sealing film for 96-well and 384-well PCR and qPCR plates. Provides optically transparent, pierceable seal with minimal autofluorescence for fluorescent detection applications. Pressure-sensitive adhesive for easy application. |
| Intended Use | Sealing PCR and qPCR plates to prevent evaporation and cross-contamination during thermal cycling, while allowing fluorescence detection through the film for real-time and endpoint optical reading. |
| Principle / Technology | Transparent polyester film with medical-grade acrylic pressure-sensitive adhesive. Optical clarity combined with low autofluorescence in visible and near-UV spectrum enables fluorescence detection through the sealed film. Adhesive maintains seal integrity through repeated thermal cycling from 4 C to 100 C. |
| Detection Method | Optical clarity measured by spectrophotometer (400-700 nm transmittance); autofluorescence testing on qPCR instrument; seal integrity by fluorescein dye leak test after 40 thermal cycles |
| Sample Type | Applied to filled PCR/qPCR plates containing DNA templates and fluorescent detection master mixes |
| Performance Range / Specifications | Transmittance >90% at 450-700 nm; autofluorescence <100 RFU across FAM, HEX, ROX, Cy5 channels; evaporation <1% over 40 PCR cycles; temperature range -20 C to 110 C |
| Sensitivity / LOD | No detectable increase in baseline fluorescence vs unsealed plate across all standard qPCR channels |
| Specificity | Adhesive does not leach PCR inhibitors; no passive dye adsorption to film surface; compatible with SYBR Green, TaqMan, and molecular beacon detection |
| Reaction Conditions / Protocol | Remove protective liner; align film over plate; apply firm pressure with applicator squeegee across entire surface; ensure complete adhesion to all well rims; trim excess if needed. |
| Components / Formulation | Polyester film base (50 um thickness), medical-grade acrylic adhesive layer (30 um), silicone-coated PET release liner |
| Storage Conditions | 15-25 C; store flat in original packaging; protect from dust and humidity |
| Shelf Life | 24 months from manufacture |
| Package Specifications | 100 sheets per box, individually interleaved with release liners; 25 sheets per small pack |
| Product Form | Pre-cut sheets sized for 96-well plate footprint (approximately 80 x 130 mm); optical clear; non-sterile |
| Quality Control | Optical transmittance scan per lot; autofluorescence measurement on qPCR instrument; adhesive peel strength; PCR evaporation test; PCR inhibitor screening |
| Key Features | Optical clear for qPCR fluorescence detection; pierceable for sample recovery; pressure-sensitive adhesive for easy application; minimal autofluorescence; DNase/RNase-free |
| Purity | DNase/RNase-free certified; PCR inhibitor-free; no detectable fluorescent compounds leaching at 95 C |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Polyester base film with medical-grade adhesive; manufactured in ISO Class 7 cleanroom under ISO 13485; silicone-coated PET liner from certified supplier |
| pH Range / Optimal pH | Adhesive stable pH 4-10; no adhesive transfer to plate surface at recommended application conditions |
| Shipping Conditions | Ambient; film sheets protected from folding, creasing, and particulate contamination |
| Expiration Date / Stability | 2 years from manufacture; adhesive properties and optical clarity maintained when stored in original packaging |
| Regulatory / Compliance | ISO 13485:2016; CE marked for IVD use; FDA registered facility; compliant with REACH and RoHS directives |
| Compatibility | Compatible with polypropylene PCR plates (non-skirted, semi-skirted, full-skirted); suitable for 96-well and 384-well format plates; compatible with all top-reading fluorescence detection instruments; pierceable with standard pipette tips for sample recovery |
| Recommended Buffer System | Compatible with all PCR master mix formulations; adhesive resistant to mineral oil overlay and common PCR additives (DMSO, betaine, BSA) |
| Application Notes / Precautions | Apply with firm, even pressure across entire plate surface; use dedicated sealing film applicator for optimal adhesion; for piercing, use a clean pipette tip and pierce film at well center to access sample post-amplification; do not reuse film sheets |
| Batch-to-Batch Consistency | Film thickness +/- 5 um; adhesive peel strength (90 degree) 200-400 g/cm per ASTM D3330; optical transmittance within +/- 3% of reference at 450 nm per batch |
For research use only, not for clinical use.
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