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ACK Red Blood Cell Lysis Buffer, Sterile

Cat.No: RERE-0223 Datasheet

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Product Details Related Products
Product Name ACK Red Blood Cell Lysis Buffer, Sterile
Catalog No. RERE-0223
Description Sterile ACK lysis buffer for removing red blood cells from single-cell suspensions, the standard ammonium chloride-potassium formulation of the erythrocyte-lysis approach. Rapid selective lysis of erythrocytes leaves leucocytes and other nucleated cells intact, which makes the buffer the routine prep step before flow cytometry, immunophenotyping and primary-cell isolation from blood or tissue. Supplied filter-sterilised; handle aseptically and keep at 2-8°C, with a 12 month shelf life unopened.
Intended Use Removal of erythrocytes from single-cell suspensions ahead of flow cytometry, immunophenotyping and primary-cell isolation
Principle / Technology Ammonium chloride-potassium formulation lyses erythrocytes selectively while sparing nucleated cells
Specificity Sterile solution; filter-sterilised; 12 month shelf life
Reaction Conditions / Protocol Add to the cell suspension at the ratio and incubation the protocol specifies, monitor lysis, then stop the reaction with excess buffer and wash before downstream use
Storage Conditions 2-8°C
Package Specifications 100 mL | 500 mL
Key Features Standard ACK formulation; filter-sterilised; 12 month shelf life

For research use only, not for clinical use.

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