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| Product Name | AccI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0377 |
| Description | This recombinant rapid endonuclease targets the degenerate site 5 prime GT^MKAC 3 prime, where M stands for A or C and K for G or T, and clears plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. Supplied at 10 U/uL, it operates in the unified rapid series buffer at 37°C and is inactivated by 20 minutes at 80°C CpG sequences fully overlapping the site block cutting, and the enzyme shares its specificity with the isoschizomers XmiI and FblI. Typical uses include constructing recombinant vectors, inserting target genes and preparing linearised plasmid templates. |
| Intended Use | Molecular cloning, vector construction and preparation of linearised plasmid templates |
| Principle / Technology | Site specific cleavage at 5 prime GT^MKAC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; isoschizomers XmiI and FblI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Single common buffer for the whole series; high enzyme activity redundancy |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Restriction fragment profile on agarose gel |
For in vitro diagnostic use.
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