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| Product Name | Acc65I Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0593 |
| Description | Part of a rapid restriction endonuclease series, Acc65I recognises the palindromic sequence 5 prime GGTACC 3 prime and cuts between the two guanines to leave a four base 5 prime overhang, completing digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. The rapid series enzymes share a single digestion buffer, which simplifies reaction setup and gives good activity redundancy for over-digestion or difficult templates. The enzyme works at 37°C and is inactivated by 5 minutes at 65°C, and Dcm or CpG methylation of the substrate blocks cleavage. Asp718I is an isoschizomer while KpnI is a neoschizomer. |
| Intended Use | Rapid DNA digestion for cloning and restriction analysis of plasmids, PCR products and genomic DNA |
| Principle / Technology | Cleavage of the 5 prime GGTACC 3 prime palindrome leaving a four base 5 prime overhang within 5 to 15 minutes |
| Sample Type | Plasmid DNA, PCR products and genomic DNA |
| Specificity | Isoschizomer Asp718I; neoschizomer KpnI; cleavage blocked by Dcm and CpG methylation |
| Reaction Conditions / Protocol | Digest in 1x rapid series buffer at 37°C for 5 to 15 minutes and heat inactivate at 65°C for 5 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 60 T |
| Key Features | Single shared buffer across the rapid series; short 5 to 15 minute digestion |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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