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| Product Name | A Media Supplement, 50×, Sterile (Aminopterin) |
| Catalog No. | RERE-0400 |
| Description | Aminopterin is the selective engine of the HAT selection scheme: it blocks the de novo pathway cells use to build nucleotides, so only lines carrying a functioning salvage pathway survive. Supplied as a sterile 50× concentrate, one 10 mL bottle fortifies half a litre of complete medium, and it pairs with the H and HT supplements to assemble the full selection system or to backfill formulas where only the aminopterin block is required. |
| Intended Use | Selection of hybridomas and other cells engineered to carry salvage-pathway competence in HAT-based schemes. |
| Principle / Technology | Aminopterin inhibits de novo nucleotide synthesis, eliminating cells unable to use the salvage route. |
| Specificity | 50× concentrate; aminopterin; sterile solution; 10 mL prepares 500 mL of medium. |
| Reaction Conditions / Protocol | Dilute 1:50 into complete medium under aseptic conditions as part of the selection system. |
| Storage Conditions | -20°C; protected from light; 12 months. |
| Package Specifications | 10 mL, 10 × 10 mL |
| Key Features | Sterile 50× format; single bottle fortifies 500 mL; complements H and HT supplements. |
For research use only, not for clinical use.
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