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| Product Name | 96-Well qPCR Plate, White, Optical Clear Film Compatible |
| Catalog No. | IGM-HMM-0042 |
| Description | Optimized white 96-well qPCR plate with precision flat optical bottom and raised sealing rims for perfect optical adhesive film bonding. Engineered specifically for real-time quantitative PCR with top-reading fluorescence detection instruments. |
| Intended Use | Quantitative gene expression analysis, SNP genotyping, pathogen detection, and copy number variation assays using SYBR Green, TaqMan, or molecular beacon probe-based qPCR chemistries. |
| Principle / Technology | White well walls reflect excitation and emission light toward the detector, increasing signal-to-noise ratio. Flat optical-grade well bottoms ensure consistent focal plane across all 96 positions. Rim geometry designed for maximum contact area with optical adhesive films. |
| Detection Method | Optical performance validated on multiple qPCR platforms; seal integrity by fluorescein leakage test; thermal uniformity by infrared camera analysis |
| Sample Type | cDNA from reverse transcription, purified genomic DNA, viral RNA after RT, microRNA cDNA, bisulfite-converted DNA for methylation analysis |
| Performance Range / Specifications | Signal-to-noise improvement >4x vs clear plates; Ct standard deviation <0.15 across 96 replicate wells; seal leakage rate <0.1% wells per plate |
| Sensitivity / LOD | Reliable detection down to single-copy template; linear dynamic range >9 log units (R2 >0.998) |
| Specificity | No well-to-well fluorescence bleed-through; ROX passive reference channel compatible |
| Reaction Conditions / Protocol | Load 10-50 uL reaction volume per well; apply optical adhesive film with applicator tool; centrifuge plate; program qPCR instrument with appropriate dye calibration. |
| Components / Formulation | TiO2-pigmented polypropylene plate body; well bottom thickness 0.25 mm +/- 0.02 mm for optimal thermal transfer |
| Storage Conditions | 15-30 C in clean, dust-free environment; protect from UV and fluorescent light |
| Shelf Life | 36 months |
| Package Specifications | 25 plates per case with optical adhesive films included (25 sheets); 100 plates bulk dispensing box |
| Product Form | Non-skirted/semi-skirted options, white qPCR plate with flat optical bottom, individually sealed |
| Quality Control | Optical bottom flatness measurement; qPCR functional test with 10-fold dilution series (10^0 to 10^9 copies); seal compatibility tested with optical adhesive films |
| Key Features | Maximized fluorescence signal; optical flat bottom for reproducible readings; raised rim design for optimal film adhesion; RNase/DNase-free certified; barcoded for LIMS integration |
| Purity | PCR-clean certified; free of DNase, RNase, human genomic DNA; ATP-free; bioburden <0.1 CFU/plate |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Specialized optical-grade polypropylene formulation; manufactured in ISO Class 7 cleanroom |
| pH Range / Optimal pH | Inert across standard PCR buffer pH range (pH 7.5-9.0); no leachates detectable by LC-MS |
| Shipping Conditions | Ambient temperature; plates cushioned and secured in rigid outer carton |
| Expiration Date / Stability | 3 years from manufacture; optical properties guaranteed when stored in original packaging |
| Regulatory / Compliance | ISO 13485:2016; CE IVDR compliant; FDA 510(k) exempt for general purpose labware; ANSI/SLAS compliant |
| Compatibility | Compatible with QuantStudio series, CFX96/CFX384, LightCycler 480/96, Rotor-Gene Q, and most top-reading qPCR instruments; optical film compatible with pressure-sensitive and heat-seal films |
| Recommended Buffer System | Validated for use with all commercial qPCR master mixes; compatible with SYBR Green I, EvaGreen, TaqMan probes, molecular beacons, and FRET probe systems |
| Application Notes / Precautions | Use dedicated optical adhesive film applicator pad to ensure bubble-free, leak-proof seal; centrifuge briefly after sealing; avoid touching well bottoms with bare hands to prevent optical interference from skin oils |
| Batch-to-Batch Consistency | Optical transmittance and reflectance monitored per batch with calibrated spectrophotometer; qPCR performance validated on 3 instrument platforms per lot release |
For research use only, not for clinical use.
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