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| Product Name | 96-Well PCR Plate, Semi-Skirted, White |
| Catalog No. | IGM-HMM-0041 |
| Description | Semi-skirted white polypropylene 96-well PCR plate combining structural rigidity for robotic handling with excellent optical properties for fluorescent detection. White walls maximize signal reflection for qPCR and fluorescence-based assays. |
| Intended Use | Quantitative real-time PCR (qPCR), endpoint fluorescence detection, and high-throughput PCR in automated workflows requiring plate-handling compatibility and enhanced optical signal. |
| Principle / Technology | White pigmented polypropylene compound optimizes fluorescence signal reflection to detector while preventing optical cross-talk between adjacent wells. Semi-skirted edge provides rigid framework for robotic gripper handling and barcode application. |
| Detection Method | Optical uniformity measured by scanning fluorimeter; thermal performance on qPCR platform; robotic handling drop-test validation |
| Sample Type | cDNA for gene expression analysis, genomic DNA for genotyping, environmental DNA samples, clinical diagnostic specimens |
| Performance Range / Specifications | Fluorescence signal enhancement 2-3x vs clear plates; well-to-well optical CV <2%; evaporation <2% over 40 qPCR cycles |
| Sensitivity / LOD | Compatible with ROX, FAM, VIC, HEX, Cy5 detection channels; no autofluorescence above background in common qPCR channels |
| Specificity | No passive dye adsorption to well walls; signal linearity R2 >0.99 across 6-log template dilution range |
| Reaction Conditions / Protocol | Dispense qPCR master mix and template; seal with optical adhesive film or optical caps; centrifuge briefly to collect contents; place in qPCR instrument. |
| Components / Formulation | White-pigmented polypropylene (TiO2 masterbatch), no fluorescent optical brighteners or UV stabilizers that could interfere |
| Storage Conditions | 15-30 C; protect from direct UV light to maintain optical properties |
| Shelf Life | 36 months |
| Package Specifications | 25 plates per case, individually sealed; 50 plates bulk case option |
| Product Form | Semi-skirted 96-well plate, white polypropylene, individually sealed in clean PE bag |
| Quality Control | Optical well-to-well uniformity scan; qPCR functional test with standard DNA template dilution series; bioburden and nuclease testing |
| Key Features | White wells enhance fluorescence signal 2-3x; semi-skirted for robotic handling; cut-corner orientation (H1); alphanumeric grid marking; raised rims for effective sealing |
| Purity | Certified DNase/RNase-free; PCR inhibitor-free; human genomic DNA free (<0.5 pg/uL detection limit) |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade white polypropylene formulation; manufactured under ISO 13485 in cleanroom environment |
| pH Range / Optimal pH | Chemically inert; TiO2 pigment fully encapsulated in polymer matrix with no detectable leaching |
| Shipping Conditions | Ambient; plates stacked with interleaving protective sheets to prevent surface scratching |
| Expiration Date / Stability | 3 years; optical properties stable throughout shelf life if stored away from UV exposure |
| Regulatory / Compliance | ISO 13485:2016; CE IVD marked; FDA 21 CFR 820 compliant; ANSI/SLAS 1-2004 footplate dimensions |
| Compatibility | Compatible with Applied Biosystems QuantStudio, Bio-Rad CFX, Roche LightCycler 480, and most qPCR platforms; robotic compatible with Tecan, Hamilton, Beckman Coulter handlers |
| Recommended Buffer System | Compatible with all commercial qPCR master mixes including SYBR Green, TaqMan probe, and intercalating dye chemistries |
| Application Notes / Precautions | Use optical adhesive sealing film for best signal performance; centrifuge plate at 1000 x g for 1 minute after sealing to eliminate bubbles; avoid scratching well bottoms which may affect optical readings |
| Batch-to-Batch Consistency | Optical reflectance within +/- 2% of reference standard across batches; thermal response verified on qPCR reference instrument per production lot |
For research use only, not for clinical use.
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