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| Product Name | 96-Well PCR Plate, Non-Skirted, Clear |
| Catalog No. | IGM-HMM-0040 |
| Description | Clear polypropylene 96-well PCR plate in standard non-skirted format. Ultra-thin uniform well walls ensure rapid and consistent thermal transfer across all 96 positions. Compatible with standard thermal cycler blocks and automated plate handlers. |
| Intended Use | High-throughput PCR amplification, DNA sequencing reactions, and thermal cycling applications in standard 0.2 mL format thermal cyclers requiring non-skirted plate geometry. |
| Principle / Technology | Precision injection-molded polypropylene with 0.2 mL well capacity. Non-skirted design provides flexibility for use with multiple thermal cycler models. Uniform well wall thickness (0.25 mm +/- 0.02 mm) ensures consistent heat transfer kinetics. |
| Detection Method | Thermal uniformity validation using thermocouple array; evaporation rate testing over 40 PCR cycles; well dimension verification by coordinate measuring machine |
| Sample Type | DNA templates, PCR master mixes, sequencing reaction components, genotyping samples |
| Performance Range / Specifications | Well-to-well temperature variation <0.3 C at 95 C; evaporation <3% after 40 cycles sealed with adhesive film; well volume 0.2 mL +/- 0.02 mL |
| Sensitivity / LOD | Minimum reaction volume 5 uL with thermal pad compression; no detectable well-to-well cross-talk |
| Specificity | Free of PCR inhibitors validated by qPCR amplification efficiency 90-110% compared to tube control |
| Reaction Conditions / Protocol | Add PCR master mix and template to wells; seal with adhesive or heat-seal film; place in thermal cycler; recommended maximum fill volume 100 uL. |
| Components / Formulation | Medical-grade polypropylene (USP Class VI), no additives, mold release agents, or surface coatings |
| Storage Conditions | 15-30 C; store in original packaging to maintain cleanliness; protect from dust and particulates |
| Shelf Life | 36 months from date of manufacture |
| Package Specifications | 25 plates per case, individually sealed in polyethylene bags; 10 plates per case option |
| Product Form | Non-skirted 96-well plate format, clear natural polypropylene, individually bagged |
| Quality Control | Well dimensional inspection per ANSI/SLAS 4-2004; thermal uniformity test on reference cycler; PCR functionality qualification per lot; DNase/RNase testing |
| Key Features | Non-skirted design for wide thermal cycler compatibility; ultra-thin walls for rapid thermal equilibration; raised well rims for effective sealing; alphanumeric well identification |
| Purity | Certified DNase-free, RNase-free, human DNA-free; endotoxin <0.01 EU/well; PCR inhibitor-free validated |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Imported medical-grade polypropylene; injection molded in ISO Class 8 cleanroom under ISO 13485 |
| pH Range / Optimal pH | Polypropylene is chemically inert; no detectable pH shift in well contents after 24h incubation at 37 C |
| Shipping Conditions | Ambient temperature; plate stacks protected by rigid outer packaging to prevent warping |
| Expiration Date / Stability | 3 years from manufacture date; performance validated throughout shelf life |
| Regulatory / Compliance | ISO 13485:2016; CE marked; compliant with ANSI/SLAS microplate standards; RoHS and REACH compliant |
| Compatibility | Compatible with Applied Biosystems, Bio-Rad, Eppendorf, and most standard 96-well thermal cyclers; compatible with automated liquid handlers and plate readers |
| Recommended Buffer System | Compatible with all standard PCR buffer formulations including Tris-based, KCl-based, and commercial master mixes |
| Application Notes / Precautions | Use plate-specific sealing film or mat for optimal sealing; ensure proper alignment in thermal cycler block; for non-skirted plates, use plate support accessory if handling manually |
| Batch-to-Batch Consistency | Well wall thickness CV <3% per mold cavity and across batches; thermal profile validated on 3 reference cycler models per batch |
For research use only, not for clinical use.
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