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| Product Name | 96-Well High-Content Screening Plate, Glass Bottom, Black Walls |
| Catalog No. | IGM-HMM-0065 |
| Description | A black-walled 96-well microplate with an optically clear glass bottom for high-resolution fluorescence and confocal microscopy-based screening applications. The glass substrate provides near-zero autofluorescence and flatness superior to plastic alternatives, while the black walls eliminate optical crosstalk between wells. The plate surface is tissue culture treated for cell attachment and the glass bottom thickness is compatible with high numerical aperture microscope objectives. |
| Intended Use | High-resolution live-cell and fixed-cell imaging in automated screening platforms requiring optical clarity and low background. |
| Principle / Technology | Glass-bottom plate format with black walls for well-isolated fluorescence detection |
| Detection Method | Automated fluorescence microscopy, confocal microscopy, high-content screening systems |
| Sample Type | Adherent mammalian cell lines cultured for high-content screening of compound libraries or siRNA collections |
| Performance Range / Specifications | Well volume: 400 µL maximum; glass thickness: 0.17 ± 0.005 mm (No. 1.5 cover glass); growth area: 0.32 cm² per well |
| Sensitivity / LOD | Autofluorescence at 488 nm: ≤5% of typical cell fluorescence signal |
| Specificity | Tissue culture treated polystyrene walls; borosilicate glass bottom compatible with all standard cell types |
| Reaction Conditions / Protocol | Seed cells at 50–200 µL per well; standard cell culture incubation conditions apply; image through glass bottom using inverted microscope with oil or air objectives |
| Components / Formulation | 96-well glass-bottom plate, black polystyrene walls, borosilicate glass bottom, TC-treated, individually wrapped sterile pouches |
| Storage Conditions | Room temperature, in sealed pouches |
| Shelf Life | 24 months |
| Package Specifications | 10 plates per case (individually wrapped) |
| Product Form | Rigid plate, individually pouched and sterile |
| Quality Control | Glass flatness verified by interferometry; cell growth tested on HeLa and U2OS cells per lot |
| Key Features | Borosilicate glass bottom thickness matched to No. 1.5 cover glass specification ensures optimal resolution with oil immersion objectives |
| Purity | Sterile (SAL 10⁻⁶); DNase/RNase free; pyrogen-free; non-cytotoxic |
| Concentration | Not applicable — solid consumable product |
| Activity / Unit Definition | Performance verified per dimensional and functional specifications |
| Molecular Weight | Not applicable — polymer/glass consumable product |
| Source / Origin | Virgin polypropylene, polystyrene, borosilicate glass as specified |
| pH Range / Optimal pH | Not applicable — solid consumable |
| Shipping Conditions | Ambient temperature in protective packaging |
| Expiration Date / Stability | 36 months in sealed packaging; sterility maintained until package opened |
| Regulatory / Compliance | ISO 13485; CE-marked; FDA registered; USP Class VI materials |
| Compatibility | Compatible with standard laboratory pipettes, thermal cyclers, and centrifuges |
| Recommended Buffer System | Not applicable — use with standard aqueous buffers and organic solvents per chemical compatibility |
| Application Notes / Precautions | Single use only for sterile products; inspect packaging integrity before use; dispose per biohazard guidelines |
| Batch-to-Batch Consistency | Dimensional tolerance within specification; functional testing per AQL sampling plan |
For research use only, not for clinical use.
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