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| Product Name | 96-Well ELISA Microplate, Medium Binding, Flat Bottom |
| Catalog No. | IGM-HMM-0046 |
| Description | Polystyrene 96-well microplate with medium-binding surface optimized for assays requiring moderate protein adsorption. Provides controlled binding with lower background for applications where excess adsorption may cause non-specific interference. |
| Intended Use | ELISA formats where moderate antibody or antigen coating density is optimal, including competitive ELISA, small molecule immunoassays, and assays using pre-coated antigens with limited binding requirements. |
| Principle / Technology | Native polystyrene surface with controlled hydrophobicity provides binding capacity of 200-300 ng IgG/cm2. Reduced surface charge density compared to high-binding plates minimizes non-specific ionic interactions while maintaining adequate protein adsorption for most immunoassay formats. |
| Detection Method | Protein binding capacity by IgG binding isotherm; uniformity by HRP-conjugated antibody ELISA; optical flatness measurement |
| Sample Type | Serum, plasma, cell culture media, tissue homogenates, purified recombinant proteins, hapten-protein conjugates |
| Performance Range / Specifications | Binding capacity 200-300 ng IgG/cm2; well-to-well CV <5%; background OD <0.050 at 450 nm in standard ELISA |
| Sensitivity / LOD | Detection sensitivity comparable to high-binding for high-affinity antibody systems; lower non-specific binding in complex sample matrices |
| Specificity | Reduced hydrophobic non-specific binding compared to high-binding surfaces; recommended for samples with high lipid or lipoprotein content |
| Reaction Conditions / Protocol | Coat wells with 100 uL per well of antigen or antibody in appropriate buffer; incubate overnight at 4 C or 2 hours at 37 C; wash; block; proceed with sample incubation and detection. |
| Components / Formulation | Crystal polystyrene, medium-binding surface (no additional chemical modification), clear non-pigmented, individual packaging with desiccant |
| Storage Conditions | 15-30 C in sealed packaging with desiccant; protect from humidity and dust |
| Shelf Life | 24 months in sealed bag with desiccant |
| Package Specifications | 5 plates per sealed bag; 100 plates per case; individual strip plate format available |
| Product Form | Flat-bottom 96-well solid or 12 x 8 strip format, medium binding, clear |
| Quality Control | IgG binding capacity 200-300 ng/cm2 specification check; background absorbance <0.050 at 450 nm; well flatness; surface uniformity |
| Key Features | Moderate binding for controlled protein adsorption; lower background than high-binding plates; ideal for competitive assays; break-apart strip format for flexible assay design |
| Purity | Binding capacity per specification; endotoxin <0.01 EU/well; tested for lot-to-lot binding uniformity |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Crystal-grade polystyrene; manufactured under ISO 13485 quality system in controlled environment |
| pH Range / Optimal pH | Binding optimal pH 7.0-9.6; plate functional pH 2-12; avoid prolonged exposure to organic solvents |
| Shipping Conditions | Ambient temperature; protect from humidity; desiccant indicator included in sealed packaging |
| Expiration Date / Stability | 24 months from manufacture date; binding stability depends on sealed, desiccated storage conditions |
| Regulatory / Compliance | ISO 13485:2016; CE IVD marked; USP <661> compliant; FDA registered facility |
| Compatibility | Fits all standard 96-well plate readers, washers, automated processors, and shaker-incubators; compatible with multichannel pipettes and robotic handlers |
| Recommended Buffer System | Coating buffers: PBS pH 7.4, carbonate buffer pH 9.6, Tris-HCl pH 8.0; blocking: BSA, casein, non-fat milk, gelatin; wash: PBST (PBS + 0.05% Tween-20) |
| Application Notes / Precautions | Medium binding is recommended when high coating density causes steric hindrance or when antigen is abundant and low background is prioritized; optimize coating concentration empirically between 1-10 ug/mL |
| Batch-to-Batch Consistency | Protein binding capacity measured per production lot by IgG saturation assay; optical uniformity verified across 96 wells per quality control plate; annual external calibration of spectrophotometric reference |
For research use only, not for clinical use.
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