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| Product Name | 96-Well ELISA Microplate, High Binding, Flat Bottom |
| Catalog No. | IGM-HMM-0045 |
| Description | Polystyrene 96-well microplate with modified high-binding surface chemistry for maximum protein adsorption capacity. Flat bottom well geometry optimized for spectrophotometric reading. Ideal for sandwich and indirect ELISA protocols requiring high coating efficiency. |
| Intended Use | Solid-phase immunoassay platform for sandwich ELISA, indirect ELISA, and protein binding assays where maximal passive adsorption of capture antibodies or antigens is required for assay sensitivity. |
| Principle / Technology | Modified polystyrene surface with increased hydrophobicity and elevated oxygen/carbon ratio promotes hydrophobic and ionic interactions with protein molecules. Surface binding capacity 400-500 ng IgG/cm2, approximately twice that of medium binding plates. |
| Detection Method | Protein binding capacity by IgG saturation assay; well-to-well CV by colorimetric ELISA; optical clarity by 450 nm transmittance measurement |
| Sample Type | Serum, plasma, cell culture supernatant, purified antibodies, recombinant antigens, urine, CSF for immunoassay |
| Performance Range / Specifications | Binding capacity 400-500 ng IgG/cm2; well-to-well absorption CV <5% for colorimetric ELISA; optical flatness <0.01 mm across well bottom |
| Sensitivity / LOD | Sub-nanogram per mL detection achievable with optimized assay design; signal-to-noise ratio >10 at 100 pg/mL antigen |
| Specificity | Low non-specific binding background; blocking buffer compatibility verified with BSA, casein, and commercial blocking formulations |
| Reaction Conditions / Protocol | Coat wells with 100 uL capture antibody in carbonate buffer pH 9.6 overnight at 4 C; wash; block; add sample and detection reagents per standard ELISA protocol. |
| Components / Formulation | Medical-grade crystal polystyrene, high-binding surface treatment, clear non-pigmented polymer, individually wrapped with desiccant |
| Storage Conditions | 15-30 C in sealed bag with desiccant; avoid humidity exposure which reduces binding capacity |
| Shelf Life | 24 months in sealed packaging with desiccant |
| Package Specifications | 5 plates per sealed bag with desiccant; 100 plates per case; individually wrapped plates per case |
| Product Form | Flat-bottom 96-well strip or solid plate, high binding, clear, sterile or non-sterile |
| Quality Control | IgG binding capacity tested per lot; optical flatness measurement; sterility per USP <71> for sterile format; DNase/RNase tested |
| Key Features | Maximum binding capacity for sensitive ELISA; low well-to-well variation; optically clear flat bottom; compatible with all microplate readers; single-well break-apart strip format available |
| Purity | Certified IgG binding capacity; low endotoxin (<0.01 EU/well); tested free of DNase/RNase |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade crystal polystyrene; surface-modified under controlled process in ISO 13485 certified facility |
| pH Range / Optimal pH | Binding optimal in pH 7.0-9.6 coating buffer; plate stable in pH 2-10 washing and incubation buffers |
| Shipping Conditions | Ambient temperature; protect from humidity with sealed desiccant packaging; plate orientation upright during transit |
| Expiration Date / Stability | 2 years from manufacture when stored in sealed bag with active desiccant; binding capacity degrades if exposed to humidity |
| Regulatory / Compliance | ISO 13485:2016; CE IVD marked; compliant with USP <661> for physicochemical testing of plastic containers |
| Compatibility | Compatible with all standard 96-well microplate readers, washers, and automated ELISA processors; strip format fits standard strip holders and frames |
| Recommended Buffer System | Coating buffer: 0.05 M carbonate-bicarbonate pH 9.6; blocking: 1-5% BSA or casein in PBS; wash: PBS with 0.05% Tween-20; stop solution: 1-2 N H2SO4 or HCl |
| Application Notes / Precautions | Do not allow wells to dry out between incubation steps; use multichannel pipette for consistent timing across plate; pre-warm plate and reagents to room temperature for optimal reproducibility |
| Batch-to-Batch Consistency | Binding capacity verified on 3-plate sample per lot; optical baseline at 450 nm <0.050 AU across all wells; CV <3% within plate and <5% plate-to-plate in lot release test |
For research use only, not for clinical use.
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