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| Product Name | 90 µm Crosslinked Agarose Mixed-Mode Ion-Exchange Media Up to 50 mg/mL |
| Catalog No. | IGM-HMM-0289 |
| Description | Alta DiagnoTech mixed-mode ion-exchange media use highly crosslinked agarose microspheres carrying multiple functional groups. Phenyl is combined with either carboxyl or quaternary ammonium functionality to provide selectivity distinct from conventional ion exchangers. |
| Intended Use | Selective charge-based separation and purification of biomolecules. |
| Principle / Technology | Mixed-mode weak cation exchange or mixed-mode strong anion exchange on crosslinked agarose. |
| Performance Range / Specifications | Two 90 µm media provide approximately 50 mg/mL lysozyme capacity or 30 mg/mL BSA capacity. |
| Product Form | Mixed-mode agarose chromatography medium |
| Key Features | Highly crosslinked agarose Mixed-mode cation and anion options 90 µm particle size Distinct selectivity from conventional ion exchange |
| Product Type | Mixed-mode ion-exchange chromatography medium |
| Function / Efficacy | Selective ion-exchange purification of biomolecules. |
| Chromatography Mode | Mixed-mode weak cation exchange or mixed-mode strong anion exchange |
| Matrix | Highly crosslinked agarose |
| Particle Size | 90 µm |
| Ligand / Bonded Phase | Phenyl/carboxyl or phenyl/quaternary ammonium |
| Dynamic Binding Capacity | Approximately 50 mg/mL for lysozyme on the cation-exchange medium; approximately 30 mg/mL for BSA on the anion-exchange medium |
| Maximum Operating Pressure | 0.3 MPa |
| CIP / Sanitization Conditions | 1 M NaOH |
| Recommended Flow Rate | 150-250 cm/h |
| pH Stability | pH 2-12 |
| Chemical Stability | Compatible with common buffers, 1 M acetic acid, 1 M NaOH, 1 M HCl, 70% ethanol, 30% isopropanol, 30% acetonitrile, 1% SDS, 6 M guanidine hydrochloride and 8 M urea; avoid strong oxidants. |
| Operating Temperature | 4-30 °C |
For research use only, not for clinical use.
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