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| Product Name | 80 µm PMMA Metal-Chelate Affinity Media with IDA, NTA or TED Ligands |
| Catalog No. | IGM-HMM-0291 |
| Description | Alta DiagnoTech immobilized-metal affinity media use highly crosslinked polyacrylate microspheres covered by a hydrophilic film and coupled with chelating groups. Uniform particles and low nonspecific adsorption support selective, high-recovery capture of tagged proteins. |
| Intended Use | Capture and purification of tagged proteins and other proteins that coordinate with immobilized transition-metal ions. |
| Principle / Technology | Immobilized-metal affinity chromatography based on coordination between Ni2+, Cu2+, Zn2+ or Co2+ and histidine, tryptophan or cysteine residues on proteins. |
| Performance Range / Specifications | Three ligand options use 80 µm PMMA particles and operate at up to 0.5 MPa across pH 2-12. |
| Storage Conditions | 20% ethanol at 4-25 °C |
| Package Specifications | 30 mL, 50 mL, 100 mL, 300 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L |
| Product Form | Immobilized-metal affinity chromatography medium |
| Key Features | 80 µm uniform particles IDA, NTA and TED chelator options Low nonspecific adsorption High mechanical and chemical stability |
| Product Type | Immobilized-metal affinity chromatography medium |
| Function / Efficacy | Metal-coordination capture of tagged and histidine-rich proteins. |
| Chromatography Mode | Immobilized-metal affinity chromatography |
| Matrix | Highly crosslinked polyacrylate microspheres with a hydrophilic surface film |
| Particle Size | 80 µm |
| Ligand / Bonded Phase | IDA, NTA or TED chelating ligand |
| Ligand Coupling Method | Chelating groups are coupled over a hydrophilic film covering the microsphere surface. |
| Maximum Operating Pressure | 0.5 MPa |
| CIP / Sanitization Conditions | 0.5 M NaOH |
| Recommended Flow Rate | 150-750 |
| pH Stability | pH 2-12 |
| Chemical Stability | When nickel is removed, compatible with stable aqueous solutions, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropanol, 6 M guanidine hydrochloride, 8 M urea and 70% ethanol. |
| Operating Temperature | 4-30 °C |
For research use only, not for clinical use.
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