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| Product Name | 75 µm Alkali-Resistant Protein A Agarose Medium ~70 mg/mL Human IgG DBC |
| Catalog No. | IGM-HMM-0230 |
| Description | The Alta DiagnoTech recombinant Protein A affinity medium uses highly crosslinked agarose beads with epoxy-coupled Protein A ligand. It is designed for purification of monoclonal, bispecific and polyclonal antibodies and recombinant proteins containing Fc fragments, with high mechanical strength, low backpressure and strong alkali tolerance. |
| Intended Use | Affinity purification of monoclonal antibodies, bispecific antibodies, polyclonal antibodies and Fc-containing recombinant proteins from laboratory through industrial scale. |
| Principle / Technology | Protein A affinity chromatography using epoxy-coupled recombinant Protein A on crosslinked agarose. |
| Performance Range / Specifications | Average particle size approximately 75 µm; dynamic binding capacity approximately 70 mg/mL for human IgG at six minutes residence time; maximum pressure 0.5 MPa. |
| Storage Conditions | 20% ethanol or 2% benzyl alcohol at 2-8 °C |
| Package Specifications | 30 mL, 50 mL, 100 mL, 300 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L |
| Product Form | Agarose affinity chromatography medium |
| Key Features | ~70 mg/mL human IgG dynamic binding capacity High-rigidity agarose matrix Low nonspecific adsorption Alkali-resistant Protein A ligand |
| Product Type | Protein A affinity chromatography medium |
| Function / Efficacy | Capture and purification of antibodies and Fc-containing recombinant proteins. |
| Chromatography Mode | Protein A affinity chromatography |
| Matrix | Highly crosslinked agarose |
| Particle Size | Approximately 75 µm |
| Ligand / Bonded Phase | Recombinant Protein A |
| Ligand Coupling Method | Epoxy coupling |
| Dynamic Binding Capacity | Approximately 70 mg/mL for human IgG at six minutes residence time |
| Maximum Operating Pressure | 0.5 MPa |
| CIP / Sanitization Conditions | 0.1-0.5 M NaOH |
| Recommended Flow Rate | Up to 500 cm/h in a 600 mm ID × 250 mm bed column |
| pH Stability | pH 3-12 during operation; pH 2-14 during CIP |
| Chemical Stability | Common buffers, 10 mM HCl, 0.1 M citric acid at pH 3, 6 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 20% ethanol |
| Operating Temperature | 2-40 °C |
For research use only, not for clinical use.
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