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| Product Name | 69 µm High-Capacity Protein A Agarose Medium ≥65 mg/mL Human IgG DBC |
| Catalog No. | IGM-HMM-0257 |
| Description | The Alta DiagnoTech Protein A affinity medium combines a mechanically reinforced agarose matrix with epoxy-coupled Protein A. The porous hydrophilic beads provide selective antibody binding, alkali-compatible cleaning and practical pressure-flow performance for downstream capture. |
| Intended Use | Affinity capture of monoclonal antibodies and other Protein A-binding immunoglobulins. |
| Principle / Technology | Protein A affinity capture on reinforced porous agarose beads with epoxy ligand coupling. |
| Performance Range / Specifications | 69 µm particles; dynamic binding capacity ≥65 mg/mL for human IgG at a six-minute residence time; maximum pressure 0.3 MPa; recommended flow 100-500 cm/h. |
| Storage Conditions | 20% ethanol or 2% benzyl alcohol at 2-8 °C |
| Package Specifications | 30 mL, 50 mL, 100 mL, 300 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L |
| Product Form | Agarose Protein A affinity chromatography medium |
| Key Features | ≥65 mg/mL human IgG dynamic binding capacity at six minutes Epoxy-coupled Protein A ligand 0.1-0.5 M NaOH cleaning tolerance 100-500 cm/h recommended flow |
| Product Type | Protein A affinity chromatography medium |
| Function / Efficacy | Selective capture and purification of monoclonal antibodies. |
| Customization | Prepacked columns are available in 7.7 × 22 mm, 16 × 25 mm and 7.7 × 100 mm formats; additional formats may be customized. |
| Chromatography Mode | Protein A affinity capture |
| Matrix | Agarose |
| Particle Size | 69 µm |
| Ligand / Bonded Phase | Protein A |
| Ligand Coupling Method | Epoxy coupling |
| Dynamic Binding Capacity | ≥65 mg/mL for human IgG at a six-minute residence time |
| Maximum Operating Pressure | 0.3 MPa |
| CIP / Sanitization Conditions | 0.1-0.5 M NaOH |
| Recommended Flow Rate | 100-500 cm/h |
| pH Stability | pH 2-12 |
| Chemical Stability | Common buffers, 10 mM HCl, 0.1 M citric acid at pH 3, 6 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 20% ethanol. |
| Operating Temperature | 2-40 °C |
For research use only, not for clinical use.
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