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| Product Name | 50× ROX Reference Dye for qPCR |
| Catalog No. | IVDRM-0066 |
| Description | 50× ROX Reference Dye for qPCR is a concentrated passive reference dye designed for real-time PCR instruments that rely on ROX normalization, including mainstream qPCR platforms. By providing a stable, reaction-independent fluorescent signal, it corrects for well-to-well variation caused by pipetting and sample loading, improving the accuracy and reproducibility of quantitative PCR data. The dye is supplied at a concentration of 25 µM and is compatible with the vast majority of qPCR instruments. |
| Intended Use | Used as a passive reference dye in real-time PCR to normalize fluorescent reporter signals and correct well-to-well variation caused by pipetting and loading differences on ROX-compatible qPCR instruments. |
| Principle / Technology | ROX serves as a passive reference fluorophore whose signal remains constant throughout the PCR run. The instrument software divides the reporter dye signal by the ROX signal in each well, canceling out non-reaction-related fluorescence fluctuations such as pipetting errors, volume differences, and optical variations, thereby improving data precision. |
| Sample Type | qPCR reaction mixtures on instruments requiring ROX reference signal normalization |
| Performance Range / Specifications | Compatible with the vast majority of real-time PCR instruments; supplied as a 50× concentrate (25 µM). |
| Storage Conditions | Store aliquoted at -25°C to -15°C, protected from light. Avoid repeated freeze-thaw cycles. For short-term use, the dye may be kept at 4°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C, protected from light. |
| Package Specifications | 1 mL 500 μL 100 μL |
| Concentration | 50× (25 µM) |
| Expiration Date / Stability | Stable for 2 years at -25°C to -15°C protected from light. Short-term storage at 4°C (e.g., up to one month) does not significantly affect performance; repeated freeze-thaw cycles may degrade the dye and should be avoided. |
| Compatibility | Compatible with most real-time PCR instruments that require ROX reference dye normalization, including mainstream qPCR platforms. |
| Application Notes / Precautions | Dilute the stock 1:10 before use (e.g., 10 μL stock + 90 μL diluent); recommended diluent: 20 mM Tris-HCl (pH 8.4), 0.1 mM EDTA, 0.01% Tween 20. Aliquot the dye upon receipt to minimize freeze-thaw cycles, which can degrade the dye and reduce activity. Short-term storage at 4°C (within about one month) does not significantly affect performance, but long-term storage should be at -25°C to -15°C. Protect from light during storage and handling. |
For research use only, not for clinical use.
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