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| Product Name | 50 µm Protein G Affinity PMMA Medium 45-55 mg/mL Human IgG DBC |
| Catalog No. | IGM-HMM-0244 |
| Description | The Alta DiagnoTech Protein G affinity medium immobilizes Protein G on an epoxy-activated PMMA matrix. Its broad binding spectrum includes strong interaction with antibody Fc regions and weaker interaction with Fab regions, supporting purification of antibodies and antibody fragments from cell culture and serum sources. |
| Intended Use | Affinity capture and purification of antibodies or antibody fragments from cell culture materials and sera from multiple species. |
| Principle / Technology | Protein G affinity capture using epoxy-immobilized Protein G on PMMA beads. |
| Performance Range / Specifications | 50 µm particles; dynamic binding capacity 45-55 mg/mL for human IgG at four minutes residence time; maximum pressure 0.8 MPa. |
| Package Specifications | 30 mL, 50 mL, 100 mL, 300 mL, 500 mL, 1 L, 5 L, 10 L, 50 L and 100 L |
| Product Form | PMMA affinity chromatography medium |
| Key Features | 45-55 mg/mL human IgG dynamic binding capacity Broad antibody binding spectrum Fc and Fab interactions 100-500 cm/h recommended flow |
| Product Type | Protein G affinity chromatography medium |
| Function / Efficacy | Capture and purification of antibodies and antibody fragments. |
| Customization | Prepacked columns are available in 7.7 × 22 mm, 16 × 25 mm and 7.7 × 100 mm formats; additional formats may be customized. |
| Chromatography Mode | Protein G affinity capture |
| Matrix | Polymethyl methacrylate (PMMA) |
| Particle Size | 50 µm |
| Ligand / Bonded Phase | Protein G |
| Ligand Coupling Method | Epoxy coupling |
| Dynamic Binding Capacity | 45-55 mg/mL for human IgG at four minutes residence time |
| Maximum Operating Pressure | 0.8 MPa |
| CIP / Sanitization Conditions | 6 M guanidine hydrochloride |
| Recommended Flow Rate | 100-500 cm/h |
| pH Stability | pH 2-10 |
| Chemical Stability | Common buffers, 20 mM sodium phosphate, 1% SDS, 6 M guanidine hydrochloride, 70% ethanol and 6 M urea; avoid prolonged contact with strong acids or bases |
| Operating Temperature | 2-40 °C |
For research use only, not for clinical use.
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