50 µm Poly(dT)20 mRNA Affinity Medium ~2 mg/mL DBC and 3 MPa Tolerance

Name: 50 µm Poly(dT)20 mRNA Affinity Medium ~2 mg/mL DBC and 3 MPa Tolerance
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Cat.No: IGM-HMM-0256 Datasheet

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Product Name	50 µm Poly(dT)20 mRNA Affinity Medium ~2 mg/mL DBC and 3 MPa Tolerance
Catalog No.	IGM-HMM-0256
Description	The Alta DiagnoTech mRNA affinity medium uses monodisperse macroporous PS-DVB beads functionalized with a poly(dT)20 ligand. A hydrophilic surface coating limits nonspecific binding, while base pairing with the mRNA poly(A) tail enables selective capture and release.
Intended Use	Affinity purification of polyadenylated mRNA from in-vitro transcription mixtures, including separation from enzymes, plasmid DNA and other reaction components.
Principle / Technology	Poly(dT)20 affinity capture through base pairing with the mRNA poly(A) tail.
Performance Range / Specifications	50 µm particles; dynamic binding capacity approximately 2 mg/mL for mRNA; maximum pressure 3 MPa; recommended flow 50-300 cm/h; pH stability 2-13.
Storage Conditions	20% ethanol at 2-8 °C
Product Form	PS-DVB poly(dT)20 affinity chromatography medium
Key Features	Approximately 2 mg/mL mRNA dynamic binding capacity Selective poly(A)-tail capture 3 MPa maximum pressure 2-65 °C operating temperature
Product Type	mRNA affinity chromatography medium
Function / Efficacy	Selective capture and release of polyadenylated mRNA.
Chromatography Mode	Poly(dT) affinity chromatography
Matrix	Polystyrene-divinylbenzene (PS-DVB)
Particle Size	50 µm
Ligand / Bonded Phase	Poly(dT)20
Dynamic Binding Capacity	Approximately 2 mg/mL for mRNA
Maximum Operating Pressure	3 MPa
CIP / Sanitization Conditions	0.1 M NaOH
Recommended Flow Rate	50-300 cm/h
pH Stability	pH 2-13
Chemical Stability	Common buffers, 1 M HCl, 2 M acetic acid, 100% ethanol, isopropanol and other common organic solvents; avoid strong oxidants, benzyl alcohol and tetrahydrofuran.
Operating Temperature	2-65 °C

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For research use only, not for clinical use.