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| Product Name | 50 µm High-Resolution Crosslinked Agarose Strong Cation-Exchange Medium |
| Catalog No. | IGM-HMM-0238 |
| Description | The Alta DiagnoTech strong cation-exchange chromatography medium uses highly crosslinked agarose beads coupled with a sulfonate ligand. It is designed for biomolecule purification with high binding capacity, favorable pressure-flow behavior and straightforward cleaning-in-place. |
| Intended Use | Intermediate or polishing purification of proteins, peptides, nucleic acids and other charged biomolecules. |
| Principle / Technology | Strong cation-exchange chromatography separates biomolecules according to surface-charge differences. |
| Performance Range / Specifications | 50 µm particles; 140-200 µmol/mL as H+ ion capacity; maximum operating pressure 0.3 MPa; high resolution, binding capacity and recovery for downstream purification. |
| Storage Conditions | 20% ethanol at 4-30 °C |
| Package Specifications | 25 mL, 100 mL, 500 mL, 1 L, 5 L, 10 L and 50 L |
| Product Form | Agarose chromatography medium |
| Key Features | High binding capacity Favorable pressure-flow performance Scalable chromatography workflow Simple CIP cleaning and sanitization |
| Product Type | Ion-exchange chromatography medium |
| Function / Efficacy | Intermediate or polishing purification of proteins, peptides, nucleic acids and other charged biomolecules. |
| Chromatography Mode | Strong cation-exchange |
| Matrix | Highly crosslinked agarose beads |
| Particle Size | 50 µm |
| Ligand / Bonded Phase | Sulfonate |
| Dynamic Binding Capacity | High dynamic binding capacity; no numerical value stated. |
| Ion Capacity | 140-200 µmol/mL as H+ |
| Maximum Operating Pressure | 0.3 MPa |
| CIP / Sanitization Conditions | 0.5 M NaOH |
| Recommended Flow Rate | 150-200 cm/h |
| Maximum Flow Rate | 400 cm/h at 0.1 MPa in a 26 mm ID × 15 cm bed column at 25 °C |
| pH Stability | pH 4-13 during operation; pH 2-14 during CIP |
| Chemical Stability | Common aqueous solutions, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol. |
| Operating Temperature | 4-40 °C |
For research use only, not for clinical use.
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