45-165 µm Weak Anion-Exchange DEAE Agarose Medium 110-160 µmol/mL
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45-165 µm Weak Anion-Exchange DEAE Agarose Medium 110-160 µmol/mL

Cat.No: IGM-HMM-0240 Datasheet

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Product Name 45-165 µm Weak Anion-Exchange DEAE Agarose Medium 110-160 µmol/mL
Catalog No. IGM-HMM-0240
Description The Alta DiagnoTech weak anion-exchange chromatography medium uses high-flow agarose beads coupled with a diethylaminoethyl (deae) ligand. It is designed for biomolecule purification with high binding capacity, favorable pressure-flow behavior and straightforward cleaning-in-place.
Intended Use Weak anion-exchange purification of proteins, peptides, nucleic acids and other charged biomolecules.
Principle / Technology Weak anion-exchange chromatography separates biomolecules according to surface-charge differences.
Performance Range / Specifications 45-165 µm particles; 110-160 µmol/mL as Cl− ion capacity; maximum operating pressure 0.3 MPa; suitable for packing laboratory and industrial chromatography columns.
Storage Conditions 20% ethanol at 4-30 °C
Package Specifications 25 mL, 100 mL, 500 mL, 1 L, 5 L, 10 L and 50 L
Product Form Agarose chromatography medium
Key Features High binding capacity
Favorable pressure-flow performance
Scalable chromatography workflow
Simple CIP cleaning and sanitization
Product Type Ion-exchange chromatography medium
Function / Efficacy Weak anion-exchange purification of proteins, peptides, nucleic acids and other charged biomolecules.
Customization Preparative columns are available with 10, 21.2, 30 or 50 mm internal diameters and 100, 150 or 250 mm lengths; additional formats may be customized.
Chromatography Mode Weak anion-exchange
Matrix High-flow agarose beads
Particle Size 45-165 µm
Ligand / Bonded Phase Diethylaminoethyl (DEAE)
Dynamic Binding Capacity High dynamic binding capacity; no numerical value stated.
Ion Capacity 110-160 µmol/mL as Cl−
Maximum Operating Pressure 0.3 MPa
CIP / Sanitization Conditions 0.5 M NaOH
Recommended Flow Rate 200-600 cm/h
Maximum Flow Rate 600 cm/h at 0.1 MPa in a 26 mm ID × 15 cm bed column at 25 °C
pH Stability pH 2-12 during operation; pH 2-14 during CIP
Chemical Stability Common aqueous solutions, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol.
Operating Temperature 4-40 °C

For research use only, not for clinical use.

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