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| Product Name | 45-165 µm Nickel-NTA IMAC Agarose Medium ~40 mg/mL DBC |
| Catalog No. | IGM-HMM-0231 |
| Description | The Alta DiagnoTech immobilized-metal affinity chromatography medium uses highly crosslinked agarose beads coupled with NTA and prechelated with nickel ions. Its hydrophilic porous structure supports rapid purification of histidine-tagged proteins, peptides and other histidine-containing biomolecules with low metal-ion leakage. |
| Intended Use | Immobilized-metal affinity purification of histidine-tagged proteins, peptides and other histidine-containing biomolecules. |
| Principle / Technology | Nickel-NTA immobilized-metal affinity chromatography. |
| Performance Range / Specifications | 45-165 µm particles; dynamic binding capacity approximately 40 mg/mL; nickel ion capacity 15 µmol/mL; maximum pressure 0.3 MPa. |
| Package Specifications | 25 mL, 100 mL, 500 mL, 1 L, 5 L and 10 L |
| Product Form | Nickel-chelated agarose chromatography medium |
| Key Features | Rapid His-tag protein purification ~40 mg/mL dynamic binding capacity Low nickel-ion leakage Broad chemical compatibility |
| Product Type | Immobilized-metal affinity chromatography medium |
| Function / Efficacy | Affinity capture of histidine-containing proteins and peptides. |
| Customization | Preparative columns are available in 10 × 100 mm, 10 × 150 mm and 10 × 250 mm formats; a 30 µm option is also available in selected prepacked-column formats. |
| Chromatography Mode | Nickel-NTA immobilized-metal affinity chromatography |
| Matrix | High-flow agarose beads |
| Particle Size | 45-165 µm |
| Ligand / Bonded Phase | NTA prechelated with Ni²+ |
| Dynamic Binding Capacity | Approximately 40 mg/mL |
| Ion Capacity | 15 µmol/mL Ni²+ |
| Maximum Operating Pressure | 0.3 MPa |
| CIP / Sanitization Conditions | 0.5 M NaOH after removal of Ni²+ |
| Recommended Flow Rate | 150 cm/h in a 50/30 column with 10 cm bed height |
| Maximum Flow Rate | 600 cm/h in a 16/20 column with 10 cm bed height |
| pH Stability | pH 3-12 during operation; pH 2-14 during CIP after removal of Ni²+ |
| Chemical Stability | Common aqueous solutions, 0.1 M HCl, 0.1 M NaOH, 2% SDS, 30% isopropanol, 6 M guanidine hydrochloride, 8 M urea and 70% ethanol after nickel removal |
| Operating Temperature | 4-30 °C |
For research use only, not for clinical use.
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